Thermo Fisher Scientific , 355 River Oaks Parkway, San Jose, California 95134, United States.
Anal Chem. 2013 Dec 17;85(24):11710-4. doi: 10.1021/ac403115c. Epub 2013 Nov 27.
Proteome coverage and peptide identification rates have historically advanced in line with improvements to the detection limits and acquisition rate of the mass spectrometer. For a linear ion trap/Orbitrap hybrid, the acquisition rate has been limited primarily by the duration of the ion accumulation and analysis steps. It is shown here that the spectral acquisition rate can be significantly improved through extensive parallelization of the acquisition process using a novel mass spectrometer incorporating quadrupole, Orbitrap, and linear trap analyzers. Further, these improvements to the acquisition rate continue to enhance proteome coverage and general experimental throughput.
蛋白质组覆盖范围和肽鉴定率历来随着质谱仪检测限和采集率的提高而提高。对于线性离子阱/轨道阱混合质谱仪，采集率主要受到离子积累和分析步骤持续时间的限制。本文表明，通过使用一种新型质谱仪对采集过程进行广泛的并行化处理，包括四极杆、轨道阱和线性阱分析仪，可以显著提高光谱采集率。此外，这些采集率的改进继续提高蛋白质组覆盖范围和整体实验通量。
