Department of Physics and Department of Chemistry, ‡Department of Chemical and Biological Engineering, and §Department of Molecular, Cellular, and Developmental Biology, University of Colorado , Boulder, Colorado, 80309, United States.
J Am Chem Soc. 2013 Dec 11;135(49):18292-5. doi: 10.1021/ja409815g. Epub 2013 Dec 2.
The spectroscopic characterization of biomolecular structures requires nanometer spatial resolution and chemical specificity. We perform full spatio-spectral imaging of dried purple membrane patches purified from Halobacterium salinarum with infrared vibrational scattering-type scanning near-field optical microscopy (s-SNOM). Using near-field spectral phase contrast based on the Amide I resonance of the protein backbone, we identify the protein distribution with 20 nm spatial resolution and few-protein sensitivity. This demonstrates the general applicability of s-SNOM vibrational nanospectroscopy, with potential extension to a wide range of biomolecular systems.
生物分子结构的光谱特性需要达到纳米级的空间分辨率和化学特异性。我们使用基于蛋白质主链酰胺 I 共振的近场光谱相衬，对从盐杆菌中纯化得到的干燥紫膜斑片进行了全空间-光谱成像，采用红外振动散射型扫描近场光学显微镜（s-SNOM）对其进行了拍摄。我们以 20nm 的空间分辨率和几个蛋白质的灵敏度识别出了蛋白质的分布。这证明了 s-SNOM 振动纳米光谱学的普遍适用性，该技术具有广泛应用于各种生物分子系统的潜力。
