Centre for Nanobiotechnology, VIT University, Vellore 632014, Tamil Nadu, India.
Centre for Nanobiotechnology, VIT University, Vellore 632014, Tamil Nadu, India.
Colloids Surf B Biointerfaces. 2014 Feb 1;114:392-7. doi: 10.1016/j.colsurfb.2013.10.034. Epub 2013 Oct 29.
Oil-in-water nanoemulsion was formulated using sesame oil, non-ionic surfactant (Tween20/Tween80) and water by ultrasound cavitation method. Development of nanoemulsion was optimized for process parameters such as surfactant type, surfactant concentration and emulsification time to obtain lower droplet diameter with greater stability. Increase in surfactant concentration and emulsification time resulted in nanoemulsion with minimized droplet diameter. Tween80 was more effective in reducing droplet size when compared to that of Tween20. Selected formulation with optimized process parameter (with oil-surfactant mixing ratio of 1:3 v/v and Tween80 as surfactant) was used for delivery of eugenol. Eugenol-loaded nanoemulsion was formulated with droplet diameter of 13 nm and was stable for more than 1 month. Sesame oil blended eugenol-loaded nanoemulsion demonstrated lower droplet size and higher stability than only-eugenol (without sesame oil) nanoemulsion. Eugenol-loaded nanoemulsion S3E3 exhibited antibacterial activity against Staphylococcus aureus. Inactivation kinetics of S. aureus showed time and concentration killing of bacteria upon treatment with S3E3 nanoemulsion. Fluorescence microscopy results demonstrated that S3E3 nanoemulsion treatment resulted in alteration of membrane permeability. In situ assessment of S3E3 in orange juice exhibited a significant reduction in the native bacteria population.
水包油纳米乳剂通过超声空化法使用芝麻油、非离子表面活性剂（吐温 20/吐温 80）和水来制备。通过优化工艺参数（如表面活性剂类型、表面活性剂浓度和乳化时间）来优化纳米乳剂的开发，以获得更小的粒径和更高的稳定性。增加表面活性剂浓度和乳化时间会导致纳米乳剂的粒径最小化。与吐温 20 相比，吐温 80 更有效地减小了粒径。选择具有优化工艺参数的配方（油-表面活性剂混合比为 1:3 v/v，吐温 80 为表面活性剂）用于 delivery 丁香酚。丁香酚负载的纳米乳剂的粒径为 13nm，稳定期超过 1 个月。芝麻油混合的丁香酚负载纳米乳剂的粒径小于仅含丁香酚（不含芝麻油）的纳米乳剂，稳定性更高。负载丁香酚的纳米乳剂 S3E3 对金黄色葡萄球菌表现出抗菌活性。金黄色葡萄球菌的失活动力学表明，S3E3 纳米乳剂处理会导致细菌的时间和浓度杀灭。荧光显微镜结果表明，S3E3 纳米乳剂处理会导致膜通透性发生变化。原位评估 S3E3 在橙汁中的应用表明，其对天然细菌种群有显著的减少作用。
