Petruczynik Anna, Tuzimski Tomasz, Plech Tomasz, Misiurek Justyna, Szalast Karolina, Szymczak Grażyna
Department of Inorganic Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland.
Department of Physical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland.
Molecules. 2019 Sep 20;24(19):3417. doi: 10.3390/molecules24193417.
Plants are an important origin of natural substances that the raw material for various pharmaceutical and therapeutic applications due to the presence of phytochemicals, such as alkaloids. Alkaloids, which are found in different plant species, possess numerous biological activities. Some alkaloids have strong cytotoxic effects on various cancer cells. The search for new drugs to treat various cancers is one of the most important challenges of modern scientific research. This study aimed to investigate of cytotoxic activity of extracts that were obtained from ; sp.; containing various alkaloids on selected cancer cell lines. The aim was also the quantification of selected alkaloids in the investigated extracts by HPLC. The analysis of alkaloids contents were performed while using HPLC in reversed phase (RP) mode using Polar RP column and mobile phase containing acetonitrile, water, and ionic liquid. The cytotoxic effect of the tested plant extracts and respective alkaloids' standards were examined while using human pharyngeal squamous carcinoma cells (FaDu), human tongue squamous carcinoma cells (SCC-25), human breast adenocarcinoma cell line (MCF-7), and human triple-negative breast adenocarcinoma cell line (MDA-MB-231). All of the investigated plant extracts possess cytotoxic activity against cancer cell lines: FaDu, SCC-25, MCF-7, and MDA-MB-231. The highest cytotoxic activity against FaDu and MDA-MB-231 cells was observed for root extract, while the highest cytotoxic activity against SCC-25 and MCF-7 cells was estimated for the root extract. There obtained significant differences in the cytotoxic activity of extracts that were obtained from the roots and herbs of and Based on these results, investigated plant extracts can be recommended for further investigations of anticancer activity.
植物是天然物质的重要来源，由于存在生物碱等植物化学物质，它们是各种药物和治疗应用的原材料。存在于不同植物物种中的生物碱具有多种生物活性。一些生物碱对各种癌细胞具有很强的细胞毒性作用。寻找治疗各种癌症的新药是现代科学研究最重要的挑战之一。本研究旨在调查从含有各种生物碱的[具体植物名1]和[具体植物名2]中获得的提取物对选定癌细胞系的细胞毒性活性。其目的还包括通过高效液相色谱法（HPLC）对所研究提取物中的选定生物碱进行定量。在使用反相（RP）模式的HPLC、极性RP柱以及含有乙腈、水和离子液体的流动相的条件下进行生物碱含量分析。使用人咽鳞状癌细胞（FaDu）、人舌鳞状癌细胞（SCC - 25）、人乳腺腺癌细胞系（MCF - 7）和人三阴性乳腺腺癌细胞系（MDA - MB - 231）来检测受试植物提取物和相应生物碱标准品的细胞毒性作用。所有研究的植物提取物对癌细胞系FaDu、SCC - 25、MCF - 7和MDA - MB - 231均具有细胞毒性活性。对FaDu和MDA - MB - 231细胞观察到最高细胞毒性活性的是[具体植物名1]根提取物，而对SCC - 25和MCF - 7细胞估计具有最高细胞毒性活性的是[具体植物名2]根提取物。从[具体植物名1]和[具体植物名2]的根和地上部分获得的提取物在细胞毒性活性方面存在显著差异。基于这些结果，所研究的植物提取物可推荐用于进一步的抗癌活性研究。
