测定玻璃体切割兔眼硅油和房水中的曲安奈德：应用玻璃体腔注射曲安奈德（肯乐® 40）的药代动力学研究。

Determination of triamcinolone acetonide in silicone oil and aqueous humor of vitrectomized rabbits' eyes: Application for a pharmacokinetic study with intravitreal triamcinolone acetonide injections (Kenalog® 40).

机构信息

Faculty of Pharmacy of the Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.

出版信息

J Pharm Biomed Anal. 2014 Feb;89:24-7. doi: 10.1016/j.jpba.2013.10.025. Epub 2013 Oct 29.

DOI:10.1016/j.jpba.2013.10.025

Abstract

A simple and accurate method including liquid-liquid extraction and protein precipitation procedures from silicone oil and aqueous humor samples followed by high-performance liquid chromatography (HPLC-UV) was developed and validated to determine the pharmacokinetic profile of triamcinolone acetonide in silicone oil and aqueous humor of rabbits' eyes submitted to the pars plana vitrectomy surgery. The method was successfully applied to quantify the drug remaining in silicone oil and aqueous humor (LOQ range of 1μg/mL). The triamcinolone acetonide remained in silicone oil and aqueous humor of vitrectomized rabbits' eyes for four weeks after the intravitreal injections.

摘要

建立并验证了一种从硅油和房水样品中提取和沉淀蛋白质的简单、准确的方法，随后进行高效液相色谱（HPLC-UV）分析，以确定曲安奈德在接受玻璃体切除术的兔眼硅油和房水中的药代动力学特征。该方法成功地应用于定量检测硅油和房水中残留的药物（LOQ 范围为 1μg/mL）。玻璃体内注射后，曲安奈德在玻璃体切割兔眼的硅油和房水中可维持 4 周。