Sericulture & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, NO. 133 Yiheng St. Dongguanzhuang Rd., Tianhe Ditrict, Guangzhou 510610, China.
Int J Mol Sci. 2013 Nov 18;14(11):22697-707. doi: 10.3390/ijms141122697.
Hyperoside is a major active constituent in many medicinal plants which are traditionally used in Chinese medicines for their neuroprotective, anti-inflammatory and antioxidative effects. The molecular mechanisms underlying these effects are unknown. In this study, quiescent ECV304 cells were treated in vitro with advanced glycation end products (AGEs) in the presence or absence of hyperoside. The results demonstrated that AGEs induced c-Jun N-terminal kinases (JNK) activation and apoptosis in ECV304 cells. Hyperoside inhibited these effects and promoted ECV304 cell proliferation. Furthermore, hyperoside significantly inhibited RAGE expression in AGE-stimulated ECV304 cells, whereas knockdown of RAGE inhibited AGE-induced JNK activation. These results suggested that AGEs may promote JNK activation, leading to viability inhibition of ECV304 cells via the RAGE signaling pathway. These effects could be inhibited by hyperoside. Our findings suggest a novel role for hyperoside in the treatment and prevention of diabetes.
桃叶珊瑚苷是许多药用植物中的一种主要活性成分，这些植物在传统中药中被用于治疗神经保护、抗炎和抗氧化作用。这些作用的分子机制尚不清楚。在这项研究中，用体外培养的静止型内皮细胞（ECV304）处理晚期糖基化终产物（AGEs），同时存在或不存在桃叶珊瑚苷。结果表明，AGEs 诱导 ECV304 细胞中 c-Jun N 末端激酶（JNK）的激活和凋亡。桃叶珊瑚苷抑制这些作用并促进 ECV304 细胞增殖。此外，桃叶珊瑚苷显著抑制 AGE 刺激的 ECV304 细胞中 RAGE 的表达，而 RAGE 的敲低抑制了 AGE 诱导的 JNK 激活。这些结果表明，AGEs 可能通过 RAGE 信号通路促进 JNK 的激活，从而抑制 ECV304 细胞的活力。这些作用可以被桃叶珊瑚苷抑制。我们的研究结果表明，桃叶珊瑚苷在糖尿病的治疗和预防中有新的作用。
