Clinical Medicine Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea.
Korean Convergence Medicine, University of Science and Technology (UST), Daejeon 34113, Korea.
Nutrients. 2019 Mar 4;11(3):546. doi: 10.3390/nu11030546.
Retinal apoptosis plays a critical role in the progression of diabetic retinopathy (DR), a common diabetic complication. Currently, the tight control of blood glucose levels is the standard approach to prevent or delay the progression of DR. However, prevalence of DR among diabetic patients remains high. Focusing on natural nutrients or herbal medicines that can prevent or delay the onset of diabetic complications, we administered an ethanol extract of the aerial portion of (OSSCE), a Chinese herbal medicine, over a period of 17 weeks to spontaneously diabetic Torii (SDT) rats. OSSCE was found to ameliorate retinal apoptosis through the regulation of advanced glycation end product (AGE) accumulation, oxidative stress, and mitochondrial function via the inhibition of NF-κB activity, in turn, through the downregulation of PKCδ, P47phox, and ERK1/2. We further demonstrated in 25 mM glucose-treated human retinal microvascular endothelial cells (HRMECs) that hyperoside (3-O-galactoside-quercetin), quercitrin (3-O-rhamnoside-quercetin), and 2″-O-acetylvitexin (8-C-(2″-O-acetyl-glucoside)-apigenin) were the active components of OSSCE that mediated its pharmacological action. Our results provide evidence that OSSCE is a powerful agent that may directly mediate a delay in the development or disease improvement in patients of DR.
视网膜细胞凋亡在糖尿病性视网膜病变（DR）的进展中起着关键作用，DR 是一种常见的糖尿病并发症。目前，严格控制血糖水平是预防或延缓 DR 进展的标准方法。然而，糖尿病患者中 DR 的患病率仍然很高。我们关注的是天然营养物质或草药，它们可以预防或延缓糖尿病并发症的发生，因此，我们用一种中药（OSSCE）的地上部分的乙醇提取物对自发性糖尿病 Torii（SDT）大鼠进行了为期 17 周的治疗。OSSCE 通过抑制 NF-κB 活性来调节晚期糖基化终产物（AGE）的积累、氧化应激和线粒体功能，从而改善视网膜细胞凋亡，进而通过下调 PKCδ、P47phox 和 ERK1/2 来实现。我们进一步在 25mM 葡萄糖处理的人视网膜微血管内皮细胞（HRMECs）中证明，OSSCE 的活性成分包括hyperoside（3-O-galactoside-quercetin）、quercitrin（3-O-rhamnoside-quercetin）和 2″-O-acetylvitexin（8-C-（2″-O-acetyl-glucoside）-apigenin），这些成分介导了其药理学作用。我们的结果提供了证据，表明 OSSCE 是一种强大的药物，可能直接延缓 DR 患者的疾病发展或改善疾病状况。
