由未成熟胚衍生的愈伤组织再生桃植株。

Regeneration of peach plants from callus derived from immature embryos.

机构信息

Tissue Culture and Molecular Genetics Laboratory and Field Crops Laboratory, BARC-West, USDA, ARS, 20705, Beltsville, MD, USA.

出版信息

Theor Appl Genet. 1985 Jun;70(3):248-51. doi: 10.1007/BF00304907.

DOI:10.1007/BF00304907

PMID:24252917

Abstract

Peach plants were repeatedly regenerated from immature embryos but not from callus derived from mature embryos. A white, nodular, highly regenerative callus was obtained when friable, primary callus from immature embryos was transferred from medium containing 4.5 μM 2,4-dichlorophenoxyacetic acid and 0.44 μM benzyladenine (BA) to media containing 0.27 μM α-naphthaleneacetic acid (NAA) and 2.2 μM BA. This callus retained its morphogenetic potential for a minimum of three subcultures. Green nodular callus, that lacked regenerative capacity, was produced from primary callus derived from mature embryos. Maximum regeneration of shoots occurred when highly regenerative callus was transferred to a medium in which the NAA concentration was reduced five times and the BA concentration was increased two times. Regenerated shoots were rooted in the dark on a medium containing 28.5 μM indoleacetic acid. Cytogenetic analysis of regenerated plants indicated that all plants were diploid, 2n = 2x = 16. Phenotypic evaluation of regenerated plants, grown under field conditions, is now in progress.

摘要

桃植株可由未成熟胚胎反复再生，但不能由成熟胚胎来源的愈伤组织再生。当易分散的幼胚初级愈伤组织从含有 4.5 μM 2,4-二氯苯氧乙酸和 0.44 μM 苄基腺嘌呤（BA）的培养基转移到含有 0.27 μM α-萘乙酸（NAA）和 2.2 μM BA 的培养基时，会得到白色、结节状、高再生性的愈伤组织。这种愈伤组织至少在三个继代培养中保留其形态发生潜力。从成熟胚胎来源的初级愈伤组织中产生缺乏再生能力的绿色结节状愈伤组织。当高再生性愈伤组织转移到 NAA 浓度降低五倍、BA 浓度增加两倍的培养基中时， shoots 的再生达到最大值。再生的 shoots 在含有 28.5 μM 吲哚乙酸的黑暗培养基中生根。对再生植株的细胞遗传学分析表明，所有植株均为二倍体，2n = 2x = 16。现在正在进行田间条件下再生植株的表型评估。