Helme R D, Andrews P V, Watson B A
Neurosci Lett. 1986 May 23;66(3):333-7. doi: 10.1016/0304-3940(86)90041-8.
We have measured plasma extravasation in response to stimulation of the rat hind footpad with wool fabric. The stimulus was delivered using a constantly weighted disc of wool rotating at 4 revolutions per minute for 30 min on the base of blisters induced by vacuum. The protein content of prestimulated blister base perfusate was 38 +/- 2 micrograms/ml in a 30-min collection. With stimulation this increased to 80 +/- 10 micrograms/ml (P less than 0.001; Student's paired t-test). No increase in plasma extravasation was observed in adult animals pretreated as neonates with capsaicin indicating that the plasma extravasation observed was neurogenically mediated. Plasma extravasation was also observed with substance P added to the perfusate. The threshold for substance P-induced plasma extravasation was 2 X 10(-11) mol/30 min. These results suggest that neurogenic inflammation induced by the mechanical stimulus of wool fabric may be mediated by substance P. This reaction may be involved in wool-induced prickle and itch noted in some individuals.
我们测量了用羊毛织物刺激大鼠后足垫后血浆外渗的情况。刺激是通过一个持续加重的羊毛圆盘来施加的，该圆盘在由真空诱导产生的水疱底部以每分钟4转的速度旋转30分钟。在30分钟的收集过程中，刺激前水疱底部灌流液的蛋白质含量为38±2微克/毫升。刺激后，该含量增加到80±10微克/毫升（P<0.001；学生配对t检验）。在用辣椒素预处理过的成年动物（幼年期接受过处理）中未观察到血浆外渗增加，这表明观察到的血浆外渗是由神经介导的。向灌流液中添加P物质时也观察到了血浆外渗。P物质诱导血浆外渗的阈值为2×10⁻¹¹摩尔/30分钟。这些结果表明，羊毛织物的机械刺激所诱导的神经源性炎症可能由P物质介导。这种反应可能与某些个体中出现的羊毛引起的刺痛和瘙痒有关。
