Louis S M, Jamieson A, Russell N J, Dockray G J
Department of Physiology, University of Liverpool, U.K.
Neuroscience. 1989;32(3):581-6. doi: 10.1016/0306-4522(89)90281-9.
Immunohistochemistry combined with retrograde tracing has been used to show that of the afferent neurons supplying the dorsomedial surface of the hind paw, approximately 30% contain substance P and 50% calcitonin gene-related peptide immunoreactivity. Stimulation of the saphenous nerve causes plasma extravasation and antidromic vasodilatation in this area of skin. The roles of calcitonin gene-related peptide and substance P released from peripheral afferent endings in mediating these effects were examined using immunoneutralization. In pilot experiments, the binding of radiolabelled peptide to the immunoglobulin fraction of calcitonin gene-related peptide and substance P antisera was characterized in quasi-physiological conditions. Systemic administration of either substance P or calcitonin gene-related peptide antibodies caused a significant decrease (P less than 0.05) in plasma extravasation measured by the Evans Blue method in response to topical application of mustard oil (0.5%) to the skin, or of capsaicin (5 microM) to the saphenous nerve. Topical application of mustard oil also produced a 52.9 +/- 5.1% increase in skin red cell flux. This increase was significantly decreased by both substance P and calcitonin gene-related peptide antibodies. The results suggest that both peptides are involved in mediating neurogenic inflammatory responses.
免疫组织化学结合逆行追踪已被用于表明，在供应后爪背内侧表面的传入神经元中，约30%含有P物质，50%含有降钙素基因相关肽免疫反应性。刺激隐神经会导致该皮肤区域的血浆外渗和逆向血管舒张。使用免疫中和法研究了外周传入末梢释放的降钙素基因相关肽和P物质在介导这些效应中的作用。在预实验中，在准生理条件下表征了放射性标记肽与降钙素基因相关肽和P物质抗血清的免疫球蛋白部分的结合。全身给予P物质或降钙素基因相关肽抗体，通过伊文思蓝法测量，对皮肤局部应用芥子油（0.5%）或对隐神经应用辣椒素（5微摩尔）引起的血浆外渗有显著降低（P小于0.05）。局部应用芥子油还使皮肤红细胞流量增加了52.9±5.1%。P物质和降钙素基因相关肽抗体均使这种增加显著降低。结果表明这两种肽都参与介导神经源性炎症反应。
