Plant Breeding Institute, Maris Lane, CB2 2LQ, Trumpington, Cambridge, England.
Theor Appl Genet. 1985 Jun;70(3):309-14. doi: 10.1007/BF00304917.
Intrachromosomal mapping studies were used to locate the positions of the genes Kr1 and Kr2, which control the crossability of wheat with Hordeum bulbosum, on chromosomes 5B and 5A, respectively. The location of Kr1 was established using the telocentric mapping technique and found to be on the long arm of chromosome 5B, distal to the centromere with a mean recombination frequency of 44.8±3.28%. Kr2 was located on the long arm of chromosome 5A by linkage with the major gene markers Vrn1, controlling vernalization requirement, and q, controlling ear morphology. Kr2 is closely linked to Vrn1, with a mean recombination frequency of 4.8±4.66%, and is distal to q with a mean recombination frequency of 38.1±10.60%. The similar locations of Kr1 and Kr2 on homoeologous chromosomes suggest that these two loci are homoeoallelic. Significant correlations between Hordeum bulbosum and rye crossability confirmed that Kr1 and Kr2 control the crossability of wheat with both species.
利用染色体内作图研究，将控制小麦与 bulbous 大麦可杂交性的 Kr1 和 Kr2 基因的位置分别定位在 5B 和 5A 染色体上。Kr1 的位置是通过着丝粒作图技术确定的，位于 5B 染色体的长臂上，靠近着丝粒，平均重组频率为 44.8±3.28%。Kr2 通过与控制春化需求的主要基因标记 Vrn1 和控制耳形态的 q 连锁定位在 5A 染色体的长臂上。Kr2 与 Vrn1 紧密连锁，平均重组频率为 4.8±4.66%，与 q 距离较远，平均重组频率为 38.1±10.60%。Kr1 和 Kr2 在同源染色体上的相似位置表明这两个位点是同效等位基因。与黑麦和大麦可杂交性的显著相关性证实，Kr1 和 Kr2 控制了小麦与这两个物种的可杂交性。
