LRM55神经胶质细胞中的一种膜蛋白与人红细胞阴离子交换载体抗体发生交叉反应。
A membrane protein in LRM55 glial cells cross-reacts with antibody to the anion exchange carrier of human erythrocytes.
作者信息
Wolpaw E W, Martin D L
出版信息
Neurosci Lett. 1986 Jun 6;67(1):42-7. doi: 10.1016/0304-3940(86)90205-3.
Cl-/HCO3-exchange transport in the glial plasma membrane is remarkably similar to anion exchange transport in erythrocytes. When LRM55 cells were pretreated with the extracellular labeling reagent [125I]iodosulfanilic acid, radioactivity appeared primarily in two bands on denaturing polyacrylamide gels at Mr 70,000 and 30,000. Antibody to the human erythrocyte anion exchanger (Band 3) cross-reacted on immunoblots with an LRM55 band at Mr 68,000. The LRM55 Cl-/HCO3-exchanger may be a component of Mr 68,000 to 70,000.
神经胶质细胞质膜中的Cl⁻/HCO₃⁻交换转运与红细胞中的阴离子交换转运非常相似。当用细胞外标记试剂[¹²⁵I]碘磺胺酸预处理LRM55细胞时，放射性主要出现在变性聚丙烯酰胺凝胶上的两条带中，分子量分别为70,000和30,000。抗人红细胞阴离子交换蛋白（带3）抗体在免疫印迹上与LRM55细胞中一条分子量为68,000的带发生交叉反应。LRM55细胞的Cl⁻/HCO₃⁻交换蛋白可能是分子量在68,000至70,000之间的一种成分。
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