Braga F R, Silva A R, Carvalho R O, Araújo J V, Pinto P S A
Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG 36570-000, Brazil.
J Helminthol. 2011 Mar;85(1):7-11. doi: 10.1017/S0022149X10000179. Epub 2010 Mar 26.
Three concentrations of chlamydospores of the nematophagous fungus Pochonia chlamydosporia (1000, 10,000 and 20,000 per Petri dish) were evaluated in vitro on Taenia taeniaeformis eggs. Chlamydospores at each concentration were cultured in two different media: 2% water-agar (2%WA) and 2% corn-meal-agar (2%CMA). Taenia taeniaeformis eggs were plated in each chlamydospore concentration in 2%WA and 2%CMA (treated groups) and without fungus (control group). Eggs were removed from each Petri dish at intervals of 7, 14 and 21 days and classified according to ovicidal activity (type 1, type 2 and type 3 effects). Plates containing 2%CMA showed the highest percentages for type 3 effect (81.3%) on the 21st day of observation. A difference (P < 0.01) between the media 2%WA and 2%CMA for type 1 effect was observed only at a concentration of 1000 chlamydospores on the 7th day. There were differences (P < 0.01) between 2%WA and 2%CMA on the 14th and 21st days, at the concentration of 20,000 chlamydospores, for type 1 and type 3 effects. Regression curves for type 3 effect in 2%WA and 2%CMA at the tested concentrations showed higher ovicidal activity with increasing chlamydospore concentrations. Results indicate that, at concentrations of 1000, 10,000 and 20,000 per Petri dish, chlamydospores of P. chlamydosporia effectively destroyed T. taeniaeformis eggs and can be considered a potential biological control agent for this cestode.
在体外评估了食线虫真菌厚垣孢子虫（Pochonia chlamydosporia）三种浓度的厚垣孢子（每个培养皿1000、10000和20000个）对豆状带绦虫（Taenia taeniaeformis）虫卵的作用。每种浓度的厚垣孢子在两种不同培养基中培养：2%水琼脂（2%WA）和2%玉米粉琼脂（2%CMA）。将豆状带绦虫虫卵接种到含有每种浓度厚垣孢子的2%WA和2%CMA中（处理组）以及不含真菌的培养基中（对照组）。每隔7、14和21天从每个培养皿中取出虫卵，并根据杀卵活性（1型、2型和3型效应）进行分类。在观察的第21天，含有2%CMA的平板显示3型效应的百分比最高（81.3%）。仅在第7天，当厚垣孢子浓度为1000个时，观察到2%WA和2%CMA培养基对1型效应存在差异（P<0.01）。在第14天和第21天，当厚垣孢子浓度为20000个时，2%WA和2%CMA在1型和3型效应方面存在差异（P<0.01）。在测试浓度下，2%WA和2%CMA中3型效应的回归曲线显示，随着厚垣孢子浓度增加，杀卵活性更高。结果表明，在每个培养皿中厚垣孢子浓度为1000、10000和20000个时，厚垣孢子虫的厚垣孢子能有效破坏豆状带绦虫虫卵，可被视为这种绦虫的潜在生物防治剂。
