Institut für Angewandte Genetik, Universität Hannover, D-3000, Hannover 21, FRG.
Theor Appl Genet. 1985 Jul;70(4):377-82. doi: 10.1007/BF00273742.
Isoelectric focusing of esterase (EST), peroxidase (PRX), and phosphoglucomutase (PGM) isozymes in 'Chinese Spring' wheat, 'Imperial' rye and several 'Chinese Spring'/'Imperial' and 'Holdfast'/'King II' addition, translocation and substitution lines revealed the chromosomal location of nine Est loci previously described and of one Prx and Pgm locus. Loci Est1, Est2, Est3, Est5, Est6 and Est7 were found on chromosome arm 5RL, Est8 and Est9 on chromosome 6R in 'Imperial' rye, and the Est10 locus on chromosome arm 4RL in 'Imperial' rye and 'King II' rye. A discrepancy was found between the chromosomal location of the Prx locus in 'Imperial' where chromosome 2R was responsible for the expression of the peroxidase enzyme, and 'King II' with chromosome 1R carrying the Prx gene. As a possible explanation, the occurrence of translocation events during the production of wheat/rye aneuploid lines is discussed. The rye Pgm locus could be associated with chromosome 4RS in 'Imperial' and 'King II' rye. Except for the location of Est loci on chromosome 5RL, the results reported in this paper lend further evidence for the assumed homoeology relationships between the chromosomes of Triticinae and for the conservation of gene synteny groups during the evolution of the Triticeae tribe.
在‘春小麦’、‘帝国黑麦’和几种‘春小麦’/‘帝国黑麦’、‘固持’/‘国王 II’添加、易位和取代系的同工酶等电聚焦中，酯酶（EST）、过氧化物酶（PRX）和磷酸葡萄糖变位酶（PGM）同工酶，揭示了先前描述的 9 个 EST 基因座和 1 个 PRX 和 PGM 基因座的染色体位置。EST1、EST2、EST3、EST5、EST6 和 EST7 基因座位于 5RL 染色体臂上，EST8 和 EST9 基因座位于‘帝国黑麦’的 6R 染色体上，EST10 基因座位于‘帝国黑麦’和‘国王 II’黑麦的 4RL 染色体臂上。在‘帝国黑麦’中，PRX 基因座的染色体位置与‘国王 II’的情况不同，后者的 1R 染色体负责过氧化物酶的表达，这表明在小麦/黑麦非整倍体系的产生过程中发生了易位事件。黑麦 PGM 基因座可能与‘帝国黑麦’和‘国王 II’黑麦的 4RS 染色体有关。除了 EST 基因座在 5RL 染色体上的位置外，本文的结果进一步证明了假定的小麦族染色体同源关系，以及在小麦族进化过程中基因同线性群的保守性。
