Entomology Department, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Hert., England.
Theor Appl Genet. 1985 Jul;70(4):407-12. doi: 10.1007/BF00273746.
A study of variation in three peptidases (PEP-3 to -5) in a parthenogenetic S. avenae field population at Rothamsted using serial one-dimensional polyacrylamide gel electrophoresis (involving changes of gel concentration and electrophoretic run-time) increased the overall number of "allozymes" (mobility variants) detected from 10 under standard conditions (6% gels, 2 h run-time) to 22, as well as revealing putative heterozygous banding patterns under some test conditions. However, an examination of another enzyme, 6-phosphogluconate dehydrogenase (6-PGD) in a sample collected at Rothamsted the following year failed, using a combination of serial methods (changes of gel concentration) and isoelectric focusing, to increase the total number of 6-PGD bands separated (seven, none of which appeared to be allelic in origin). Nevertheless, some major bands were split into several bands, whilst other infrequent bands were either gained or lost. The findings are briefly discussed.
采用连续一维聚丙烯酰胺凝胶电泳（涉及凝胶浓度和电泳运行时间的变化）研究罗瑟斯塔特部分孤雌生殖燕麦雀麦田间种群中三种肽酶（PEP-3 至 -5）的变异性，将标准条件下（6%凝胶，2 小时运行时间）检测到的“同工酶”（迁移变异体）总数从 10 个增加到 22 个，并且在某些测试条件下还揭示了潜在的杂合带型。然而，第二年在罗瑟斯塔特采集的样本中，另一种酶 6-磷酸葡萄糖酸脱氢酶（6-PGD）的检查，使用连续方法（凝胶浓度变化）和等电聚焦的组合，未能增加 6-PGD 分离带的总数（七个，没有一个似乎是等位基因起源）。尽管如此，一些主要带被分裂成几个带，而其他不常见的带则被获得或丢失。简要讨论了这些发现。
