State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium.
Arch Insect Biochem Physiol. 2021 Jan;106(1):e21752. doi: 10.1002/arch.21752. Epub 2020 Oct 20.
The grain aphid, Sitobion avenae, is an economically important cereal pest worldwide. Aphid saliva plays an essential role in the interaction between aphids and their host plants. However, limited information is available regarding the proteins found in the saliva of S. avenae. Here, the watery saliva proteins from S. avenae were collected in an artificial diet and identified using a liquid chromatography-mass spectrometry/mass spectrometry analysis. A total of 114 proteins were identified in S. avenae saliva, including several enzymes, binding proteins, and putative effectors, as well as other proteins with unknown functions. In comparison with salivary proteins from nine other aphid species, the most striking feature of the salivary protein from S. avenae was the different patterns of protein functions. Several orthologous proteins secreted by other aphid species such as glucose dehydrogenase, elongation factors, and effector C002 were also detected in S. avenae saliva and speculated to play a significant role in aphid-plant interactions. These results provide further insight into the molecular basis between aphids and cereal plant interactions.
麦长管蚜是一种世界性的重要经济作物害虫。蚜虫唾液在蚜虫与其宿主植物的相互作用中起着至关重要的作用。然而，关于麦长管蚜唾液中的蛋白质，目前的信息还很有限。在这里，我们使用液相色谱-质谱/质谱分析收集了麦长管蚜人工饲料中的水分唾液蛋白，并进行了鉴定。在麦长管蚜唾液中鉴定出了 114 种蛋白质，包括几种酶、结合蛋白和假定的效应子，以及其他具有未知功能的蛋白质。与其他 9 种蚜虫的唾液蛋白相比，麦长管蚜唾液蛋白最显著的特征是蛋白质功能模式的不同。其他蚜虫分泌的一些同源蛋白，如葡萄糖脱氢酶、延伸因子和效应子 C002，也在麦长管蚜唾液中被检测到，并推测在蚜虫-植物相互作用中发挥了重要作用。这些结果为蚜虫与谷类植物相互作用的分子基础提供了进一步的深入了解。
