Alkhedir Hussein, Karlovsky Petr, Mashaly Ashraf Mohamed Ali, Vidal Stefan
Agricultural Entomology, Georg-August-University Göttingen, Grisebachstrasse 6, 37077, Göttingen, Germany. Molecular Phytopathology and Mycotoxin Research, Georg-August-University Göttingen, Grisebachstrasse 6, 37077, Göttingen, Germany.
Molecular Phytopathology and Mycotoxin Research, Georg-August-University Göttingen, Grisebachstrasse 6, 37077, Göttingen, Germany.
Environ Entomol. 2015 Oct;44(5):1358-66. doi: 10.1093/ee/nvv114. Epub 2015 Jul 21.
Aphids have developed symbiotic associations with different bacterial species, and some morphological and molecular analyses have provided evidence of the host relationship between the primary symbiotic bacteria (Buchnera aphidicola) and the aphid while the contrary with the secondary symbiotic bacteria. In this study, we investigated the phylogenetic relationships of the bacterial endosymbionts in the aphid Sitobion avenae (F.). We characterized all bacterial endosymbionts in 10 genetically defined S. avenae clones by denaturing gradient gel electrophoresis and, from these clones, sequenced the 16S rRNA genes of both the primary endosymbiont, B. aphidicola (for the first time), and the secondary endosymbionts, Regiella insecticola and Hamiltonella defensa (for the first time). The phylogenetic analysis indicated that Buchnera from Sitobion related to those in Macrosiphoni. The analysis of the secondary endosymbionts indicated that there is no host relationship between H. defensa and R. insecticola from Sitobion and those from other aphid species. In this study, therefore, we identified further evidence for the relationship between Buchnera and its host and reported a relationship within the secondary endosymbionts of S. avenae from the same country, even though there were no relationships between the secondary bacteria and their host. We also discussed the diversity within the symbiotic bacteria in S. avenae clones.
蚜虫已与不同的细菌物种形成了共生关系，一些形态学和分子分析为主要共生细菌（蚜虫内共生菌）与蚜虫之间的宿主关系提供了证据，而对于次要共生细菌则不然。在本研究中，我们调查了麦长管蚜（F.）中细菌内共生体的系统发育关系。我们通过变性梯度凝胶电泳对10个基因定义的麦长管蚜克隆中的所有细菌内共生体进行了表征，并从这些克隆中对主要内共生体蚜虫内共生菌（首次）以及次要内共生体昆虫雷吉氏菌和防御哈氏菌（首次）的16S rRNA基因进行了测序。系统发育分析表明，来自麦长管蚜的蚜虫内共生菌与来自长管蚜属的那些相关。对次要内共生体的分析表明，来自麦长管蚜的防御哈氏菌和昆虫雷吉氏菌与来自其他蚜虫物种的那些之间不存在宿主关系。因此，在本研究中，我们为蚜虫内共生菌与其宿主之间的关系找到了进一步的证据，并报道了来自同一国家的麦长管蚜次要内共生体之间的关系，尽管次要细菌与其宿主之间不存在关系。我们还讨论了麦长管蚜克隆中共生细菌的多样性。
