Glück R, Matthieu J M, Wegmann A, Méan F
Neurochem Pathol. 1986 Apr;4(2):69-75. doi: 10.1007/BF03160186.
The encephalitogenic potential of rabies vaccines prepared from nervous tissue is a result of the presence of myelin basic protein. Vaccines prepared from duck embryos are economical and efficient, but, occasionally, cases of allergic encephalomyelitis have been reported. An improved rabies vaccine has been developed that contains the classical Pitman Moore strain of rabies virus grown in embryonated duck eggs. This vaccine has been highly purified and enriched in immunologically effective rabies virus glycoprotein antigen. We have searched for the presence of myelin basic protein using sensitive radioimmunological and immunoblotting techniques. Whereas the classical duck embryo rabies vaccine contained small amounts of myelin basic protein, in the improved purified duck embryo rabies vaccine, none could be detected.
由神经组织制备的狂犬病疫苗的致脑炎潜力是髓鞘碱性蛋白存在的结果。由鸭胚制备的疫苗经济高效，但偶尔有过敏性脑脊髓炎病例的报道。一种改进的狂犬病疫苗已被开发出来，它含有在鸭胚中培养的经典皮特曼-摩尔狂犬病病毒株。这种疫苗已被高度纯化，并富含免疫有效的狂犬病病毒糖蛋白抗原。我们使用灵敏的放射免疫和免疫印迹技术来检测髓鞘碱性蛋白的存在。经典的鸭胚狂犬病疫苗含有少量髓鞘碱性蛋白，而在改进的纯化鸭胚狂犬病疫苗中，则检测不到。
