Kuwahara S S, Burgoyne G H
Health Lab Sci. 1978 Oct;15(4):201-5.
While studying variations amony immunizing potentials of rabies vaccines, we were led to consider the possibility that the antigen in the vaccine derived from duck embryos may have been degraded by enzymes arising from host tissues. Such an enzyme, a neuraminidase, was found in duck embryo rabies vaccine through the application of a periodate-linked thiobarbituric acid assay. This acitvity could not be found in two other rabies vaccines derived from tissue cultures. We have thus concluded that there is a strong possibility that a modified antigen is present in duck embryo vaccine. In addition, the injection of this vaccine will introduce a neuraminidase which may produce localized effects in the tissues of the recipient.
在研究狂犬病疫苗免疫潜力的差异时，我们开始考虑疫苗中源自鸭胚的抗原可能已被宿主组织产生的酶降解的可能性。通过应用高碘酸盐连接硫代巴比妥酸测定法，在鸭胚狂犬病疫苗中发现了这样一种酶，即神经氨酸酶。在另外两种源自组织培养的狂犬病疫苗中未发现这种活性。因此我们得出结论，鸭胚疫苗中很可能存在修饰过的抗原。此外，注射这种疫苗会引入一种神经氨酸酶，它可能会在接受者的组织中产生局部影响。
