Botanisches Institut der Universität, Menzinger Strasse 67, D-8000, München 19, Germany.
Planta. 1984 Sep;162(3):215-9. doi: 10.1007/BF00397442.
The incorporation of [1-(3)H] geranylgeranyl diphosphate (GGPP), [1-(3)H] geranylgeranyl monophosphate (GGMP) and [U-(14)C] phytyl diphosphate (PhPP) into chlorophylls a and b in growing tobacco cell cultures was investigated. The substrates were effectively incorporated into chlorophylls a and b, 3.2% of the total activity of applied GGPP or GGMP and 12.4% of the total activity of applied PhPP being found in chlorophylls a and b after 24 h incubation. The radioactivity was found in phytyl chlorophyllide through-out which means effective hydrogenation of the alcohol moiety in the case of GGPP and GGMP. With increasing substrate concentration, the specific radioactivity of chlorophyll increased up to a saturation level which was reached either at 20-40 μM PhPP or at 60 μM GGPP and GGMP. The specific radioactivity of the chlorophyll formed during the 24-h incubation period was the same as that of the applied substrate at saturating substrate concentration. The specific radioactivity of chlorophyll a was higher than that of chlorophyll b only in the case of PhPP.
在生长的烟草细胞培养物中，研究了[1-(3)H]香叶基香叶基二磷酸（GGPP）、[1-(3)H]香叶基单磷酸（GGMP）和[U-(14)C]植基二磷酸（PhPP）掺入叶绿素 a 和 b 的情况。在 24 小时孵育后，发现这些底物有效地掺入了叶绿素 a 和 b 中，应用的 GGPP 或 GGMP 的总活性的 3.2%和应用的 PhPP 的总活性的 12.4%存在于叶绿素 a 和 b 中。放射性物质存在于叶绿醇叶绿素中，这意味着在 GGPP 和 GGMP 的情况下，醇部分得到有效氢化。随着底物浓度的增加，叶绿素的比放射性增加到饱和水平，在 20-40 μM PhPP 或 60 μM GGPP 和 GGMP 时达到饱和水平。在 24 小时孵育期间形成的叶绿素的比放射性与饱和底物浓度下应用的底物的比放射性相同。只有在 PhPP 的情况下，叶绿素 a 的比放射性才高于叶绿素 b。
