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Growth and division of single cells of higher plants in vitro.高等植物单细胞的体外生长与分裂
J Gen Physiol. 1960 Mar;43(4):841-51. doi: 10.1085/jgp.43.4.841.
2
Clonal growth in vitro of human cells with fibroblastic morphology; comparison of growth and genetic characteristics of single epithelioid and fibroblast-like cells from a variety of human organs.具有成纤维细胞形态的人细胞在体外的克隆生长；来自多种人体器官的单个上皮样细胞和成纤维细胞样细胞的生长及遗传特征比较。
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1
Cloning, Transformation and Expression of Human Interferon α2b Gene in Tobacco Plant (Nicotiana tabacum cv. xanthi).人干扰素α2b基因在烟草植株（烟草品种黄花烟草）中的克隆、转化与表达
Jundishapur J Nat Pharm Prod. 2012 Summer;7(3):111-6. Epub 2012 Aug 25.
2
[The effect of colored light on growth and composition of plant tissue cultures].[有色光对植物组织培养生长及成分的影响]
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3
[Light-color and differentiation of plastids in cell cultures of Nicotiana tabacum var. "Samsun"].[烟草品种“Samsun”细胞培养物中质体的浅色与分化]
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4
Further studies on the growth and differentiation of single, isolated cells of tobacco in vitro.进一步研究烟草单细胞在体外的生长和分化。
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[Activation of thiamine biosynthesis in callus cultures of Nicotiana Tabacum in the light].[光照下烟草愈伤组织培养中硫胺素生物合成的激活]
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The effect of hormones on anthocyanin accumulation in cell cultures of Haplopappus gracilis.激素对糙苏细胞培养物中花色苷积累的影响。
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Plating of isolated tobacco mesophyll protoplasts on agar medium.在琼脂培养基上平板培养分离的烟草叶肉原生质体。
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[Influence of light and medium on the plating efficiency of isolated cells from callus cultures of Nicotiana tabacum var. "Samsun"].[光照和培养基对烟草品种“萨姆松”愈伤组织培养物中分离细胞平板效率的影响]
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本文引用的文献

1
Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects.体细胞哺乳动物细胞遗传学。III. 来自人类和动物受试者的整倍体细胞的长期培养。
J Exp Med. 1958 Dec 1;108(6):945-56. doi: 10.1084/jem.108.6.945.

高等植物单细胞的体外生长与分裂

Growth and division of single cells of higher plants in vitro.

作者信息

BERGMANN L

出版信息

J Gen Physiol. 1960 Mar;43(4):841-51. doi: 10.1085/jgp.43.4.841.

DOI:10.1085/jgp.43.4.841
PMID:13799400
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2195034/
Abstract

The cultivation of single cells of Nicotiana tabacum L. var. "Samsun" and Phaseolus vulgaris L. var. "Early Golden Cluster" on a thin agar layer in Petri dishes is described. Under these conditions about 20 per cent of the cells divided repeatedly and established tissue clones which could be isolated and maintained as growing tissue cultures. It was possible also to follow the successive divisions of isolated cells and to observe their behavior during cytogenesis under the microscope.

摘要

本文描述了在培养皿中的薄琼脂层上对烟草“萨姆松”变种和菜豆“早金簇”变种的单细胞进行培养的方法。在这些条件下，约20%的细胞反复分裂并形成了组织克隆，这些克隆可以分离出来并作为生长的组织培养物进行维持。还可以追踪分离细胞的连续分裂过程，并在显微镜下观察它们在细胞发生过程中的行为。