高等植物单细胞的体外生长与分裂
Growth and division of single cells of higher plants in vitro.
作者信息
BERGMANN L
出版信息
J Gen Physiol. 1960 Mar;43(4):841-51. doi: 10.1085/jgp.43.4.841.
Abstract
The cultivation of single cells of Nicotiana tabacum L. var. "Samsun" and Phaseolus vulgaris L. var. "Early Golden Cluster" on a thin agar layer in Petri dishes is described. Under these conditions about 20 per cent of the cells divided repeatedly and established tissue clones which could be isolated and maintained as growing tissue cultures. It was possible also to follow the successive divisions of isolated cells and to observe their behavior during cytogenesis under the microscope.
摘要
本文描述了在培养皿中的薄琼脂层上对烟草“萨姆松”变种和菜豆“早金簇”变种的单细胞进行培养的方法。在这些条件下，约20%的细胞反复分裂并形成了组织克隆，这些克隆可以分离出来并作为生长的组织培养物进行维持。还可以追踪分离细胞的连续分裂过程，并在显微镜下观察它们在细胞发生过程中的行为。
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本文引用的文献
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