Yoshida M, Karasawa N, Ito M, Sakai M, Nagatsu I
Neurosci Res. 1986 May;3(4):356-63. doi: 10.1016/0168-0102(86)90027-1.
An antiserum was raised in a rabbit by immunization with taurine bound to bovine serum albumin. The antibody was purified by passage over an immunoabsorbent column (formyl-cellulofine-taurine) and it did not cross-react significantly with glutamate, aspartate, glycine, GABA (0.4%), glutamine, proline, cysteine, beta-alanine, cysteic acid, carnosine or homocarnosine in enzyme-linked immunosorbent assays and nitrocellulose paper immunoblots. Immunocytochemical studies employing the peroxidase-antiperoxidase immunohistochemical technique revealed that many cerebellar Purkinje cells showed taurine-like immunoreactivity. Labelled axons could be followed within the white matter up to the deep cerebellar nuclei, where numerous puncta were observed. Immunoelectron microscopic examination revealed that labelled puncta were presynaptic terminals, and axo-dendritic or axo-somatic symmetrical synapses were observed on deep cerebellar nuclear neurons.
通过用与牛血清白蛋白结合的牛磺酸免疫家兔制备了抗血清。抗体通过免疫吸附柱（甲酰纤维素-牛磺酸）纯化，并且在酶联免疫吸附测定和硝酸纤维素纸免疫印迹中，它与谷氨酸、天冬氨酸、甘氨酸、γ-氨基丁酸（0.4%）、谷氨酰胺、脯氨酸、半胱氨酸、β-丙氨酸、磺基丙氨酸、肌肽或高肌肽没有明显交叉反应。采用过氧化物酶-抗过氧化物酶免疫组织化学技术的免疫细胞化学研究表明，许多小脑浦肯野细胞呈现牛磺酸样免疫反应性。标记的轴突可在白质中追踪至小脑深部核团，在那里观察到大量的点状结构。免疫电子显微镜检查显示，标记的点状结构是突触前终末，并且在小脑深部核神经元上观察到轴突-树突或轴突-胞体对称性突触。
