在烟草属和颠茄属体细胞杂种中新型 rDNA 重复单元。
Novel class of rDNA repeat units in somatic hybrids between Nicotiana and Atropa.
机构信息
Institute of Botany, Academy of Sciences of the Ukrainian SSR, Repina 2, 252601, Kiev, USSR.
出版信息
Theor Appl Genet. 1988 Jul;76(1):108-12. doi: 10.1007/BF00288839.
Behavior of ribosomal RNA genes in the process of somatic hybridization was analyzed using hybrids Nicotiana tabacum + Atropa belladonna. Blothybridization of parental species DNAs to (32)P-rDNA specific probes revealed two classes of ribosomal repeats in both tobacco and nightshade; their length was 11.2 kb, 10.4 kb (tobacco) and 9.4 kb, 10.2 kb (night-shade). For analysis of hybrids, labelled (32)P rDNA specific probes were hybridized to DNA of parental species and somatic hybrids digested with restriction endonucleases EcoR1, EcoRV and BamH1. A new class of ribosomal DNA repeat, absent in parental species, was found in hybrid line NtAb-1. Possible mechanisms of appearence of a new rDNA class in the process of somatic cell fusion are discussed.
利用杂种烟草+颠茄进行体细胞杂交，分析核糖体 RNA 基因在体细胞杂交过程中的行为。双亲种 DNA 与（32）P-rDNA 特异性探针的 Blothybridization 显示烟草和茄属植物中存在两类核糖体重复序列；它们的长度分别为 11.2kb、10.4kb（烟草）和 9.4kb、10.2kb（茄属植物）。为了分析杂种，用标记的（32）P rDNA 特异性探针与亲本种和用限制内切酶 EcoR1、EcoRV 和 BamH1 消化的体细胞杂种 DNA 杂交。在杂种系 NtAb-1 中发现了一种新的核糖体 DNA 重复序列，在亲本种中不存在。讨论了体细胞融合过程中出现新 rDNA 类的可能机制。
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