Vegetable Crops Department, IFAS, University of Florida, 32611, Gainesville, FL, USA.
Plant Cell Rep. 1984 Jun;3(3):112-5. doi: 10.1007/BF02441013.
Leaf, shoot-tip, stem, and root explants of sweet potato (Ipomea batatas Poir.) gave rise to two kinds of callus on nutrient agar medium containing 0.5 to 2.0 mg/l 2,4-D. One callus, bright- to pale-yellow, was compact and organized, while the other was dull-yellow and friable. The former callus gave rise to numerous globular and heart-shaped embryoids. When transferred onto hormone-free medium, the embryoids readily developed into a torpedo-shape before germination. The plantlets were transplanted to soil where they flowered and formed storage roots at maturity.
甘薯(Ipomea batatas Poir.)的叶片、茎尖、茎和根外植体在含有 0.5 至 2.0mg/l 2,4-D 的营养琼脂培养基上产生了两种愈伤组织。一种愈伤组织呈亮至浅黄色,致密且有组织,而另一种呈暗黄色且易碎。前者的愈伤组织产生了许多球形和心形的胚状体。当转移到无激素的培养基上时,胚状体在萌发前很容易发育成鱼雷形。将植株移植到土壤中,成熟时它们会开花并形成块根。