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甘薯(Ipomea batatas Poir.)组织培养中的体细胞胚胎发生和植株再生。

Somatic embryogenesis and plant regeneration in tissue cultures of sweet potato (Ipomea batatas Poir.).

机构信息

Vegetable Crops Department, IFAS, University of Florida, 32611, Gainesville, FL, USA.

出版信息

Plant Cell Rep. 1984 Jun;3(3):112-5. doi: 10.1007/BF02441013.

DOI:10.1007/BF02441013
PMID:24253438
Abstract

Leaf, shoot-tip, stem, and root explants of sweet potato (Ipomea batatas Poir.) gave rise to two kinds of callus on nutrient agar medium containing 0.5 to 2.0 mg/l 2,4-D. One callus, bright- to pale-yellow, was compact and organized, while the other was dull-yellow and friable. The former callus gave rise to numerous globular and heart-shaped embryoids. When transferred onto hormone-free medium, the embryoids readily developed into a torpedo-shape before germination. The plantlets were transplanted to soil where they flowered and formed storage roots at maturity.

摘要

甘薯(Ipomea batatas Poir.)的叶片、茎尖、茎和根外植体在含有 0.5 至 2.0mg/l 2,4-D 的营养琼脂培养基上产生了两种愈伤组织。一种愈伤组织呈亮至浅黄色,致密且有组织,而另一种呈暗黄色且易碎。前者的愈伤组织产生了许多球形和心形的胚状体。当转移到无激素的培养基上时,胚状体在萌发前很容易发育成鱼雷形。将植株移植到土壤中,成熟时它们会开花并形成块根。

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本文引用的文献

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Theor Appl Genet. 1981 Sep;59(5):269-73. doi: 10.1007/BF00264978.
采用中心复合试验设计分析外源多胺和生长调节剂对甘薯原生质体培养效率的影响。
Plant Cell Rep. 1988 May;7(3):216-9. doi: 10.1007/BF00269328.
4
Improved culture conditions for somatic embryogenesis from Asparagus officinalis L. using an aseptic ventilative filter.使用无菌通气过滤器改善芦笋体细胞胚胎发生的培养条件。
Plant Cell Rep. 1991 Aug;10(5):230-4. doi: 10.1007/BF00232564.
5
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Plant Cell Rep. 1994 May;13(8):437-41. doi: 10.1007/BF00231962.
6
Rapid and repetitive plant regeneration in sweetpotato via somatic embryogenesis.甘薯体细胞胚胎发生的快速和重复的植物再生。
Plant Cell Rep. 1996 Feb;15(6):381-5. doi: 10.1007/BF00232059.
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In Vitro Cell Dev Biol Plant. 1998 Oct-Dec;34(4):310-8. doi: 10.1007/BF02822740.