School of Biological Sciences, University of Nebraska-Lincoln, 68588-0118, Lincoln, Nebraska, USA.
Plant Cell Rep. 1984 Aug;3(4):151-5. doi: 10.1007/BF00270211.
Protoplasts were prepared from suspension cultures of Nicotiana tabacum cv Wisconsin 38 that had been prelabeled with FITC. The protoplasts were subjected to flow sorting based on fluorescence content using a Coulter EPICS V Flow Cytometer - Cell Sorter. Conditions were established that allowed the recovery after sorting of approximately 30% of the initial protoplasts in a viable state. These were subsequently regenerated into calli that underwent shoot morphogenesis.
从预先用 FITC 标记的烟草悬浮培养细胞中制备原生质体。使用 Coulter EPICS V 流式细胞仪-细胞分选仪根据荧光含量对原生质体进行流式分选。建立了条件，使得在可存活状态下大约可以回收 30%的初始原生质体。这些随后被再生为愈伤组织，经历芽形态发生。
