Department of Infection Control (6901) , Copenhagen University Hospital (Rigshospitalet), Juliane Maries Vej 28, 2100, Copenhagen, OE, Denmark,
Eur J Clin Microbiol Infect Dis. 2014 May;33(5):809-13. doi: 10.1007/s10096-013-2018-2. Epub 2013 Nov 20.
Environmental sampling in hospitals is becoming increasingly important because of the rise in nosocomial infections. In order to monitor and track these infections and optimize cleaning and disinfection, we need to be able to locate the fomites with the highest amount of microorganisms, but the optimal method for this is not clear. The aim of this study was to evaluate which of four different dipslides or a standard TSA contact plate were best at recovering human bacteria from the environment. We tested four different dipslides with selective and non-selective agars versus a standard TSA contact plate in order to find the best sampling media. Two hundred sites in a children's medical ward in Copenhagen University hospital were sampled in autumn 2012. There was no difference in total bacteria count between the TSA contact plate and the dipslides. Faecal indicator bacteria recovery was the same for the dipslides and the TSA contact plate. Dipslides may be equally well suited for environmental sampling and hygiene assessment as TSA contact plates. Dipslides have some advantages, such as better sample security, easier sampling in confined spaces and longer shelf life that may speak in favour of choosing these for bacteria environmental sampling in hospitals, depending on the task.
由于医院感染的上升，医院环境采样变得越来越重要。为了监测和跟踪这些感染，并优化清洁和消毒，我们需要能够找到微生物数量最多的病原体，但最佳方法尚不清楚。本研究旨在评估四种不同的浸碟或标准 TSA 接触平板中，哪一种最能从环境中回收人体细菌。我们用选择性和非选择性琼脂测试了四种不同的浸碟，与标准 TSA 接触平板进行比较，以找到最佳的采样介质。2012 年秋季，在哥本哈根大学医院的儿科病房中对 200 个地点进行了采样。TSA 接触平板和浸碟之间的总细菌计数没有差异。浸碟和 TSA 接触平板对粪便指示菌的回收率相同。浸碟可能与 TSA 接触平板一样适合环境采样和卫生评估。浸碟具有一些优势，例如更好的样品安全性、在狭小空间中更易于采样以及更长的保质期，这可能取决于任务，有利于选择这些浸碟进行医院细菌环境采样。
