Research Laboratory, Maruzen Kasei Co., Ltd., Mukaihigashi, 722, Onomichi, Japan.
Plant Cell Rep. 1984 Oct;3(5):183-5. doi: 10.1007/BF00270195.
Clonal propagation of Stevia rebaudiana has been established by culturing stem-tips with a few leaf primordia on an agar medium supplemented with a high concentration (10 mg/l) of kinetin. Anatomical examination has suggested that these multiple shoots originate from a number of adventitious buds formed on the margin of the leaf. Innumerable shoots can be obtained by repeating the cycle of multiple-shoot formation from a single stem-tip of Stevia. These shoots produce roots when transferred to a medium containing NAA (0.1 mg/l) without kinetin. The regenerated plantlets can be transplanted to soil.
甜菊的克隆繁殖是通过在添加高浓度（10 毫克/升）激动素的琼脂培养基上培养带有少量叶原基的茎尖来实现的。解剖检查表明，这些多芽是由叶片边缘形成的多个不定芽发育而来的。通过从甜菊的单个茎尖重复多芽形成的循环，可以获得无数的芽。当这些芽转移到含有 NAA（0.1 毫克/升）而不含激动素的培养基中时，它们会生根。再生的幼苗可以移植到土壤中。
