Laboratoire d'Amelioration des Plantes, Université de Paris Sud, F-91405, Orsay, France.
Plant Cell Rep. 1985 Feb;4(1):7-11. doi: 10.1007/BF00285493.
Somatic hybridization of Lycopersicon peruvianum and Petunia hybrida was carried out to transfer cytoplasmic male sterility from Petunia to Lycopersicon. Cytological, morphological and biochemical analyses were performed to characterize the regenerated plants. Two regenerated plants, R3 and R6, were male sterile. R3 possessed chromosomes morphologically similar to those of both parental types. Leaf morphologies of these two plants and a third plant, R7, were intermediate between the two parents. The stability of RUBPCase was verified during parental plant development and after in vitro culture. Plant R7 presented a new form of the large subunit of RUBPCase.
将秘鲁番茄和矮牵牛进行体细胞杂交，以将矮牵牛的细胞质雄性不育转移到番茄中。对再生植株进行了细胞学、形态学和生化分析，以鉴定其特性。有两个再生植株，R3 和 R6，是雄性不育的。R3 具有与双亲类型相似的染色体形态。这两个植株和第三个植株 R7 的叶片形态在双亲之间是中间型的。在亲本植物发育过程中和体外培养后，验证了 RUBPCase 的稳定性。植株 R7 呈现出 RUBPCase 大亚基的一种新形式。
