Projektgruppen Haploide in der Pflanzenzüchtung, Max-Planck-Institut für Pflanzengenetik, Rosenhof, D-6802, Ladenburg, Rederal Republic of Germany.
Planta. 1980 Jul;149(2):112-7. doi: 10.1007/BF00380870.
Using somatic hybrid cell lines Arabidopsis thaliana+Brassica campestris, obtained by cloning individual protoplast-fusion products as starting material, shoots and flowering plants have been regenerated. Cytological, biochemical, and morphological analyses indicate that genetic material of both species is present in the resultant plants. Shoots and plants obtained from different lines and different regeneration events differed morphologically and genetically. Most regenerants show morphological abnormalities and unusual organizational patterns. Flowering forms have so far been sterile. "Asymmetric" hybrids (i.e., hybrids bearing most genetic material of one of the parent species and only few chromosomes of the other) were more regular in morphology. The results represent the first case of intergeneric-intertribal hybridization of flowering plants.
利用体细胞杂种细胞系拟南芥+芸薹属,以克隆的单个原生质体融合产物作为起始材料,已经再生出芽和开花植物。细胞学、生物化学和形态学分析表明,两种物种的遗传物质都存在于所得植物中。从不同的品系和不同的再生事件中获得的芽和植物在形态和遗传上都有所不同。大多数再生体表现出形态异常和不寻常的组织模式。开花形式迄今不育。“不对称”杂种(即,携带一个亲本物种的大部分遗传物质,而只有另一个亲本物种的少数染色体的杂种)在形态上更为规则。这些结果代表了开花植物属间-族间杂交的首例。