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“拟南芥-油菜”:一种通过原生质体融合获得的新型植物。

"Arabidobrassica": A novel plant obtained by protoplast fusion.

机构信息

Projektgruppen Haploide in der Pflanzenzüchtung, Max-Planck-Institut für Pflanzengenetik, Rosenhof, D-6802, Ladenburg, Rederal Republic of Germany.

出版信息

Planta. 1980 Jul;149(2):112-7. doi: 10.1007/BF00380870.

DOI:10.1007/BF00380870
PMID:24306240
Abstract

Using somatic hybrid cell lines Arabidopsis thaliana+Brassica campestris, obtained by cloning individual protoplast-fusion products as starting material, shoots and flowering plants have been regenerated. Cytological, biochemical, and morphological analyses indicate that genetic material of both species is present in the resultant plants. Shoots and plants obtained from different lines and different regeneration events differed morphologically and genetically. Most regenerants show morphological abnormalities and unusual organizational patterns. Flowering forms have so far been sterile. "Asymmetric" hybrids (i.e., hybrids bearing most genetic material of one of the parent species and only few chromosomes of the other) were more regular in morphology. The results represent the first case of intergeneric-intertribal hybridization of flowering plants.

摘要

利用体细胞杂种细胞系拟南芥+芸薹属,以克隆的单个原生质体融合产物作为起始材料,已经再生出芽和开花植物。细胞学、生物化学和形态学分析表明,两种物种的遗传物质都存在于所得植物中。从不同的品系和不同的再生事件中获得的芽和植物在形态和遗传上都有所不同。大多数再生体表现出形态异常和不寻常的组织模式。开花形式迄今不育。“不对称”杂种(即,携带一个亲本物种的大部分遗传物质,而只有另一个亲本物种的少数染色体的杂种)在形态上更为规则。这些结果代表了开花植物属间-族间杂交的首例。

相似文献

1
"Arabidobrassica": A novel plant obtained by protoplast fusion.“拟南芥-油菜”:一种通过原生质体融合获得的新型植物。
Planta. 1980 Jul;149(2):112-7. doi: 10.1007/BF00380870.
2
"Arabidobrassica": Chromosomal recombination and morphogenesis in asymmetric intergeneric hybrid cells.“拟南芥-油菜”:不对称属间杂种细胞中的染色体重组和形态发生。
Planta. 1981 Dec;153(6):586-93. doi: 10.1007/BF00385546.
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Eur J Cell Biol. 1980 Jun;21(2):227-8.
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Theor Appl Genet. 1996 Dec;93(8):1242-50. doi: 10.1007/BF00223456.

引用本文的文献

1
"Arabidobrassica": Chromosomal recombination and morphogenesis in asymmetric intergeneric hybrid cells.“拟南芥-油菜”:不对称属间杂种细胞中的染色体重组和形态发生。
Planta. 1981 Dec;153(6):586-93. doi: 10.1007/BF00385546.
2
Somatic hybridization in Nicotiana: Restoration of photoautotrophy to an albino mutant with defective plastids.体细胞杂交在烟草中的应用:用有缺陷的质体恢复白化突变体的光合作用。
Planta. 1981 Dec;153(6):497-503. doi: 10.1007/BF00385532.
3
Intertribal hybrid cell lines of Atropa belladonna (X) Nicotiana chinensis obtained by cloning individual protoplast fusion products.

本文引用的文献

1
Selection of somatic hybrids after fusion of protoplasts from Datura innoxia Mill. and Atropa belladonna L.曼陀罗和颠茄原生质体融合后体细胞杂种的选择
Planta. 1979 Jan;145(4):371-5. doi: 10.1007/BF00388363.
2
Nutrient requirements of suspension cultures of soybean root cells.大豆根细胞悬浮培养的营养需求。
Exp Cell Res. 1968 Apr;50(1):151-8. doi: 10.1016/0014-4827(68)90403-5.
3
Induction of directional chromosome elimination in somatic cell hybrids.体细胞杂种中定向染色体消除的诱导。
通过克隆单个原生质体融合产物获得的颠茄(X)烟草的部落间杂交细胞系。
Theor Appl Genet. 1982 Mar;62(1):75-9. doi: 10.1007/BF00276287.
4
Genetic processes in intergeneric cell hybrids Atropa + Nicotiana : 1. Genetic constitution of cells of different clonal origin grown in vitro.属间细胞杂种阿特拉津+烟草中的遗传过程:1. 在体外生长的不同克隆来源细胞的遗传组成。
Theor Appl Genet. 1983 Jun;65(4):269-76. doi: 10.1007/BF00276562.
5
Fraction I protein analysis of parasexual hybrid plants Arabidopsis thaliana + Brassica campestris.拟南芥+芸薹属植物的种间杂种的 I 部分蛋白分析。
Plant Cell Rep. 1981 Dec;1(2):67-8. doi: 10.1007/BF00269275.
6
Increasing the variability of Lycopersicon peruvianum Mill. by protoplast fusion with Petunia hybrida L.通过原生质体融合增加秘鲁番茄(Lycopersicon peruvianum Mill.)的变异性与矮牵牛(Petunia hybrida L.)。
Plant Cell Rep. 1985 Feb;4(1):7-11. doi: 10.1007/BF00285493.
7
Growth in vitro of arrested embryos from lethal mutants ofArabidopsis thaliana.拟南芥致死突变体胚胎的体外生长。
Theor Appl Genet. 1986 Aug;72(5):577-86. doi: 10.1007/BF00288994.
8
Regeneration of somatic hybrid plants formed between Lycopersicon esculentum and Solatium rickii.体细胞杂种再生植株的形成,该杂种是由番茄和马铃薯杂交得到的。
Theor Appl Genet. 1986 Apr;72(1):59-65. doi: 10.1007/BF00261455.
9
Individual selection, culture and manipulation of higher plant cells.高等植物细胞的个体选择、培养和操作。
Theor Appl Genet. 1987 Apr;73(6):769-83. doi: 10.1007/BF00289379.
10
Plant regeneration from protoplasts of common buckwheat (fagopyrum esculentum).普通荞麦(苦荞麦)原生质体植株再生。
Plant Cell Rep. 1989 Apr;8(4):247-50. doi: 10.1007/BF00778544.
Nature. 1971 Apr 9;230(5293):367-9. doi: 10.1038/230367a0.