Lehrstuhl für Pharmazeutische Biologie der Universität München, Karlstraße 29, D-8000, München 2, FRG.
Plant Cell Rep. 1985 Apr;4(2):96-9. doi: 10.1007/BF00269216.
Fumaria capreolata was taken into cell suspension culture. The culture yielded a biomass of about 12 g dry weight per liter of medium; the dried cells contained ca. 0.1% of alkaloids. Besides choline, the following ten known isoquinoline alkaloids were isolated from the cell extract in crystalline form: coptisine, dehydrocheilanthifoline; (+)-isoboldine; magnoflorine; N-methylcoclaurine; (+)-reticuline; (-)-pallidine; protopine; sanguinarine; (-)-scoulerine. This is the most diverse isoquinoline alkaloid spectrum thus far published for a cell suspension culture.
紫堇被接入细胞悬浮培养体系中。该培养体系每升培养基可产生约 12 克干重的生物质；干燥的细胞中大约含有 0.1%的生物碱。除了胆碱以外，从细胞提取物中还分离得到了另外十种以结晶形式存在的已知异喹啉生物碱：黄连碱、脱氢二千金藤碱；（+）-异波尔定碱；蝙蝠葛碱；N-甲基刻叶紫堇灵；（+）-网叶番荔枝碱；（-）-罂粟碱；原阿片碱；血根碱；（-）-白屈菜红碱。这是迄今为止在细胞悬浮培养物中发表的异喹啉生物碱谱中种类最多的一次。
