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由 long term haploid suspension cultures of N. plumbaginifolia 的原生质体再生植物。

Plant regeneration from protoplasts of long term haploid suspension cultures of N. plumbaginifolia.

机构信息

Applied Biosciences, Unilever Research Laboratory, Colworth House, MK44 1LQ, Sharnbrook, Bedford, UK.

出版信息

Plant Cell Rep. 1985 Apr;4(2):104-7. doi: 10.1007/BF00269218.

DOI:10.1007/BF00269218
PMID:24253696
Abstract

A predominantly haploid cell suspension culture of N.plumbaginifolia has been established. The ploidy has remained stable for nearly four years in culture (and is similar in cells recovered after preservation in liquid nitrogen). Protoplasts isolated from these cells regenerate into plants with a high frequency.

摘要

已经建立了一个主要为单倍体的 N.plumbaginifolia 细胞悬浮培养物。在培养中，ploidy 近四年来一直保持稳定（并且在液氮保存后回收的细胞中相似）。从这些细胞中分离出的原生质体以高频率再生为植物。

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本文引用的文献

1
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Planta. 1980 Jun;149(1):7-18. doi: 10.1007/BF00386221.
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Hormone-induced caulogenesis in long-term tobacco cell lines and its effect on nuclear DNA content.激素诱导的长期烟草细胞系愈伤组织发生及其对核 DNA 含量的影响。
Theor Appl Genet. 1981 Jan;60(1):31-6. doi: 10.1007/BF00275174.
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Variation in nuclear DNA content of isonicotinic acid hydrazide-resistant cell lines and mutant plants of Nicotiana tabacum.
在烟草雀稗冠瘿组织中转移 DNA 的结构、组织和表达。
Planta. 1987 Jul;171(3):393-405. doi: 10.1007/BF00398685.
4
Effect of repeated DNA sequences on direct gene transfer in protoplasts of Nicotiana plumbaginifolia.重复 DNA 序列对烟草原生质体直接基因转移的影响。
Theor Appl Genet. 1989 Jul;78(1):113-8. doi: 10.1007/BF00299763.
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Efficient induction by amiprophos-methyl and flow-cytometric sorting of micronuclei in Nicotiana plumbaginifolia.利用胺丙畏高效诱导以及通过流式细胞术分选白花烟草中的微核。
Planta. 1987 Dec;172(4):473-8. doi: 10.1007/BF00393862.
6
Isolation and characterization of microprotoplasts from APM-treated suspension cells ofNicotiana plumbaginifolia.从 APM 处理的烟草悬浮细胞中分离和表征微原生质体。
Theor Appl Genet. 1991 Sep;82(3):346-52. doi: 10.1007/BF02190621.
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Direct cell to cell transfer of organelles by microinjection.通过显微注射实现细胞器的直接细胞间转移。
Plant Cell Rep. 1992 Jan;10(12):613-6. doi: 10.1007/BF00232381.
8
A comparison of the effects of various spindle toxins on metaphase arrest and formation of micronuclei in cell-suspension cultures ofNicotiana plumbaginifolia.比较各种纺锤体毒素对白花烟草悬浮细胞中期阻滞和微核形成的影响。
Planta. 1990 Oct;182(3):408-14. doi: 10.1007/BF02411392.
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Seedling lethality in Nicotiana plumbaginifolia conferred by Ds transposable element insertion into a plant-specific gene.Ds转座元件插入植物特异性基因导致的碧冬茄幼苗致死性。
Plant Mol Biol. 2002 Oct;50(3):551-62. doi: 10.1023/a:1019851913083.
10
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Plant Cell. 2001 May;13(5):1095-107.
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4
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5
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Theor Appl Genet. 1983 Jul;66(1):67-75. doi: 10.1007/BF00281851.
6
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Plant Cell Rep. 1982 Apr;1(3):104-7. doi: 10.1007/BF00272364.
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Plant Physiol. 1970 Apr;45(4):372-5. doi: 10.1104/pp.45.4.372.