• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

原生质体衍生细胞集落和悬浮培养物的根瘤农杆菌转化。

Transformation of protoplast-derived cell colonies and suspension cultures by Agrobacterium tumefaciens.

机构信息

Unilever Research, Colworth House, MK44 1LQ, Sharnbrook, Bedfordshire, UK.

出版信息

Plant Cell Rep. 1985 Aug;4(4):202-5. doi: 10.1007/BF00269289.

DOI:10.1007/BF00269289
PMID:24253882
Abstract

In this paper we describe procedures for transforming micro colonies derived from mesophyll protoplasts of Petunia hybrida with Agrobacterium tumefaciens. The method is efficient, up to 70% of the colonies were transformed, and we used a similar method to transform cells from a suspension culture of haploid Nicotiana plumbaginifolia.

摘要

本文描述了用根癌农杆菌转化矮牵牛叶肉原生质体微菌落的方法。该方法效率高，可达 70%的菌落被转化，我们还用类似的方法转化了来自烟草孤雌生殖细胞悬浮培养物的细胞。

相似文献

1
Transformation of protoplast-derived cell colonies and suspension cultures by Agrobacterium tumefaciens.原生质体衍生细胞集落和悬浮培养物的根瘤农杆菌转化。
Plant Cell Rep. 1985 Aug;4(4):202-5. doi: 10.1007/BF00269289.
2
Shoot regeneration of mesophyll protoplasts transformed by Agrobacterium tumefaciens, not achievable with untransformed protoplasts.农杆菌转化的叶肉原生质体的再生，未转化的原生质体无法实现。
Theor Appl Genet. 1986 Apr;72(1):135-40. doi: 10.1007/BF00261469.
3
Vacuum infiltration of Petunia hybrida pollen with Agrobacterium tumefaciens to achieve plant transformation.用根癌农杆菌对矮牵牛花粉进行真空渗透以实现植物转化。
Plant Cell Rep. 2000 Jul;19(8):792-797. doi: 10.1007/s002990050009.
4
The mutagenic effect of gamma rays on leaf protoplasts of haploid and dihaploid Nicotiana plumbaginifolia, estimated by valine resistance mutation frequencies.用缬氨酸抗性突变频率估算γ射线对烟草（普通烟）单倍体和二倍体叶原生质体的诱变效应。
Theor Appl Genet. 1985 Jun;70(3):259-64. doi: 10.1007/BF00304909.
5
Flow cytometric analysis and sorting of plant chromosomes from Petunia hybrida protoplasts.矮牵牛原生质体植物染色体的流式细胞仪分析与分选
Cytometry. 1987 Sep;8(5):500-8. doi: 10.1002/cyto.990080511.
6
Agarose plating and a bead type culture technique enable and stimulate development of protoplast-derived colonies in a number of plant species.琼脂糖平板法和珠型培养技术能够促进和刺激许多植物中原生质体衍生菌落的发育。
Plant Cell Rep. 1983 Oct;2(5):244-7. doi: 10.1007/BF00269151.
7
Targeted mutagenesis using the Agrobacterium tumefaciens-mediated CRISPR-Cas9 system in common wheat.利用农杆菌介导的 CRISPR-Cas9 系统在普通小麦中进行靶向诱变。
BMC Plant Biol. 2018 Nov 26;18(1):302. doi: 10.1186/s12870-018-1496-x.
8
Establishment of an efficient genetic transformation method in Dunaliella tertiolecta mediated by Agrobacterium tumefaciens.利用根癌农杆菌介导建立高效的杜氏盐藻遗传转化方法。
J Microbiol Methods. 2018 Jul;150:9-17. doi: 10.1016/j.mimet.2018.05.010. Epub 2018 May 16.
9
T-DNA-insert-independent mutations induced in transformed plant cells during Agrobacterium co-cultivation.农杆菌共培养过程中在转化植物细胞中诱导产生的不依赖于T-DNA插入的突变。
Transgenic Res. 1994 Sep;3(5):317-25. doi: 10.1007/BF01973592.
10
Fertile indica and japonica rice plants regenerated from protoplasts isolated from embryogenic haploid suspension cultures.由胚性单倍体悬浮培养物分离的原生质体再生出可育的籼稻和粳稻植株。
Plant Cell Rep. 1992 Dec;12(1):45-9. doi: 10.1007/BF00232422.

引用本文的文献

1
Transformation of carrot tissues derived from proembryogenic suspension cells: A useful model system for gene expression studies in plants.由原胚性悬浮细胞衍生的胡萝卜组织的转化：用于植物基因表达研究的有用模型系统。
Plant Mol Biol. 1987 May;8(3):265-74. doi: 10.1007/BF00015034.
2
High efficiency Agrobacterium-mediated transformation of Lycopersicon based on conditions favorable for regeneration.基于有利于再生的条件实现番茄的高效农杆菌介导转化。
Plant Cell Rep. 1987 Apr;6(2):105-8. doi: 10.1007/BF00276664.
3
Genetic transformation in two potato cultivars with T-DNA from disarmed Agrobacterium.

本文引用的文献

1
In vitro transformation of petunia cells by an improved method of co-cultivation with A. tumefaciens strains.用改良的根癌农杆菌共培养方法进行矮牵牛细胞的体外转化。
Plant Mol Biol. 1984 Nov;3(6):371-8. doi: 10.1007/BF00033384.
2
Nutritional requirements of protoplast-derived, haploid tobacco cells grown at low cell densities in liquid medium.在液体培养基中低细胞密度生长的原生质体衍生的烟草单倍体细胞的营养需求。
Planta. 1980 Jun;149(1):7-18. doi: 10.1007/BF00386221.
3
The toxicity of antibiotics to plant cell cultures.抗生素对植物细胞培养物的毒性。
用脱武装的农杆菌 T-DNA 对两个马铃薯品种进行遗传转化。
Theor Appl Genet. 1987 Sep;73(5):744-50. doi: 10.1007/BF00260785.
4
Stable transformation of eggplant (Solanum melongena L.) by cocultivation of tissues withAgrobacterium tumefaciens carrying a binary plasmid vector.利用携带二元质粒载体的根癌农杆菌共培养组织稳定转化茄子（Solanum melongena L.）。
Plant Cell Rep. 1989 Jun;8(6):370-3. doi: 10.1007/BF00716677.
5
Transformation of pea (Pisum sativum L.) byAgrobacterium tumefaciens.豌豆（Pisum sativum L.）的根癌农杆菌转化。
Plant Cell Rep. 1989 Jun;8(6):321-4. doi: 10.1007/BF00716664.
6
Electroporation stimulates tranformation of freshly isolated cell suspension protoplasts ofSolanum dulcamara byAgrobacterium.电穿孔刺激刚分离的悬液原生质体转化为甜叶菊的根癌农杆菌。
Plant Cell Rep. 1989 Feb;8(2):86-9. doi: 10.1007/BF00716845.
Plant Cell Rep. 1983 Feb;2(1):36-9. doi: 10.1007/BF00269232.
4
Plant regeneration from protoplasts of long term haploid suspension cultures of N. plumbaginifolia.由 long term haploid suspension cultures of N. plumbaginifolia 的原生质体再生植物。
Plant Cell Rep. 1985 Apr;4(2):104-7. doi: 10.1007/BF00269218.
5
The effects of amino acids and ammonium on the growth of plant cells in suspension culture.氨基酸和铵对悬浮培养植物细胞生长的影响。
Plant Physiol. 1970 Apr;45(4):372-5. doi: 10.1104/pp.45.4.372.
6
Conditioning of the host cell as a factor in the transformation process in crown gall.宿主细胞的预处理作为冠瘿瘤转化过程中的一个因素。
Growth. 1952 Jun;16(2):65-74.
7
Crown gall transformation of tobacco callus cells by cocultivation with Agrobacterium tumefaciens.通过与根癌土壤杆菌共培养实现烟草愈伤组织细胞的冠瘿转化。
Biochem Biophys Res Commun. 1984 Sep 17;123(2):458-62. doi: 10.1016/0006-291x(84)90252-3.
8
DNA-mediated gene transfer is more efficient during S-phase of the cell cycle.
Biochem Biophys Res Commun. 1984 Jan 13;118(1):310-6. doi: 10.1016/0006-291x(84)91102-1.
9
Expression of bacterial genes in plant cells.细菌基因在植物细胞中的表达。
Proc Natl Acad Sci U S A. 1983 Aug;80(15):4803-7. doi: 10.1073/pnas.80.15.4803.
10
Crown gall tumorigenesis. II. Relations between wound healing and the tumorigenic response.冠瘿瘤形成。II. 伤口愈合与肿瘤发生反应之间的关系。
Cancer Res. 1966 Aug;26(8):1597-605.