Laboratorium voor Teeltfysiologie en Tropische Fytotechnie K. U. Leuven, K. Mercierlaan, 92, B-3030, Leuven (Heverlee), Belgium.
Plant Cell Rep. 1985 Apr;4(2):108-11. doi: 10.1007/BF00269219.
Somatic embryogenesis of Cichorium intybus L. var. 'Carolus' is induced using cubical pieces of mature tap roots with an intervening callus phase. A Murashige and Skoog's (MS) semi solid basal medium supplemented with 2,4-dichlorophenoxyacetic acid (0.02 or 0.2 mg/l) and benzylaminopurine (0.25 mg/l) and a liquid MS medium devoid of growth regulators are used respectively for induction of callus and somatic embryoids and for further development and germination. Regeneration from the nodular proembryonal stage to the full grown embryoids occurs following different morphological pathways depending on the physical and chemical environment of the culture. Further development of these embryos into plantlets and the possibilities of application of this technique in plantbreeding have been discussed.
菊苣（Cichorium intybus L. var. 'Carolus'）体细胞胚胎发生采用成熟块根的立方块诱导，中间有愈伤组织阶段。Murashige 和 Skoog（MS）半固体基础培养基分别添加 0.02 或 0.2 mg/l 2,4-二氯苯氧乙酸和 0.25 mg/l 苯并氨基嘌呤用于诱导愈伤组织和体细胞胚状体，以及液体 MS 培养基不含生长调节剂用于进一步发育和萌发。从结节原胚阶段到完全成熟胚状体的再生取决于培养的物理和化学环境，会出现不同的形态途径。已经讨论了这些胚胎进一步发育成小植株的可能性以及该技术在植物育种中的应用。
