从 S. chacoense Bitt. 的花药离体培养和 S. tuberosum L. x S. chacoense Bitt. 的种间双倍体杂种的离体培养中进行植株再生。

Plant regeneration from in vitro culture of anthers of Solanum chacoense Bitt. and interspecific diploid hybrids S. tuberosum L. x S. chacoense Bitt.

机构信息

International Plant Research Institute, 830 Bransten Road, 94070, San Carlos, CA, USA.

出版信息

Theor Appl Genet. 1984 Dec;69(2):139-43. doi: 10.1007/BF00272886.

Abstract

Production of plants from cultured anthers of Solanum chacoense clone IP 33, of its interspecific diploid hybrids with S. tuberosum clones IP 354 and IP 372, and of a complex Solanum hybrid containing in its genome S. ajanhuiri is reported. Genotypic differences were found to influence both the induction phase and the regeneration process. Hybrids derived from clone IP 354 of S. tuberosum were much more responsive in culture than hybrids from clone IP 372. Altogether, 507 plants were regenerated and 309 were cytologically analyzed. Of these, 52% were haploid, 47% diploid and 1% mixoploid or tetraploid. A number of diploid plants probably originated from unreduced microspores and some genetic consequences of this event are discussed.

摘要

本文报道了从栽培的 S. chacoense 无性系 IP 33 的花药、与其 S. tuberosum 无性系 IP 354 和 IP 372 的种间双体杂种以及含有 S. ajanhuiri 基因组的复杂 S. 杂种植物的生产。发现基因型差异既影响诱导阶段又影响再生过程。来自 S. tuberosum 无性系 IP 354 的杂种在培养中比来自 IP 372 的杂种反应更为强烈。总共再生了 507 株植物，其中 309 株进行了细胞学分析。其中，52%为单倍体，47%为二倍体，1%为混倍体或四倍体。一些二倍体植物可能来自未减数的小孢子，讨论了这一事件的一些遗传后果。