Abteilung Straub, Max-Planck-Institut für Züchtungsforschung (Erwin-Baur-Institut), Köln, Federal Republic of Germany.
Theor Appl Genet. 1979 Mar;55(2):49-55. doi: 10.1007/BF00285189.
The techniques of microspore and protoplast regeneration starting from dihaploid Solanum tuberosum plants has been improved to such an extent that the production of more than 2000 microspore derived A1 plant lines and of several hundred protoplast derived plantlets has become possible. Further, from the dihaploid Solanum species S. phureja the regeneration of microspores to plants, and from the species S. infundibuliforme, S. sparsipilum and S. tarijense the regeneration of protoplasts to calluses, has been achieved. The plants descending from the two single cell culture systems are compared with reference to phenotypic markers and economic qualities. Some principles characteristic for either microspore or protoplast derived plants are examined and their significance is discussed. The results are compiled into an extended analytical synthetic breeding scheme based on a stepwise reduction of the autotetraploid to the monohaploid level and a subsequent controlled combination to a new synthetic completely heterozygous tetraploid potato.
从二倍体马铃薯小孢子和原生质体再生植株的技术已得到极大的改进，使得产生超过 2000 株小孢子衍生的 A1 植株系和几百株原生质体衍生的植株成为可能。此外，从二倍体马铃薯种薯 S. phureja 中再生小孢子为植株，从 S. infundibuliforme、S. sparsipilum 和 S. tarijense 中再生原生质体为愈伤组织，已经成为可能。从这两个单细胞培养系统衍生的植株，就表型标记和经济性状进行了比较。考察了一些对小孢子或原生质体衍生植株有特征性的原理，并讨论了其意义。结果被整理成一个扩展的分析综合育种方案，该方案基于逐步将同源四倍体降低到单倍体水平，然后进行有控制的组合，形成一个新的完全异源杂合四倍体马铃薯。
