Projektgruppen Haploide in der Pflanzenzüchtung, Max-Planck-Institut für Pflanzengenetik, Ladenburg-Rosenhof.
Theor Appl Genet. 1977 Mar;51(2):81-6. doi: 10.1007/BF00299481.
Further progress of studies aimed at increasing production of androgenetic Secale cereale plants via the culture of anthers is described. Two culture media initially developed for rice and wheat anther culture have been shown to have pronounced influence on rye. It has been possible to increase the average percentages of responsive anthers (i.e. those producing embryoids or calluses) from 0.26% to 10% with a maximum in certain experiments of over 40 %. Of nearly 400 plants produced in 1976, 1/4 are green and can be grown further by transfer to potting compost; 3/4 are albino. Stable green haploid lines were present amongst the plants, and after vegetative propagation of the lines representative samples have been treated with colchicine resulting in diploid, triploid and tetraploid plants. The influence of the genetic background of the donor plants on the success rate of anther culture and on the percentage of albino formation is discussed.
进一步发展通过花药培养生产雄性生殖的黑麦草的研究工作描述如下。最初为水稻和小麦花药培养而开发的两种培养基对黑麦表现出明显的影响。通过这种方法，可使响应性花药（即产生胚状体或愈伤组织的花药）的平均百分率从 0.26%提高到 10%，在某些实验中最高可达 40%以上。在 1976 年生产的近 400 株植物中，有 1/4 是绿色的，可以通过转移到盆栽混合基质中进一步生长；3/4 是白化的。在这些植物中存在稳定的绿色单倍体系，并且在对系进行营养繁殖之后，用秋水仙素处理代表性样本，导致形成二倍体、三倍体和四倍体植物。讨论了供体植物的遗传背景对花药培养成功率和白化形成百分率的影响。
