Department of Genetics, University of California, Berkeley, California, USA.
Theor Appl Genet. 1978 Sep;51(5):217-22. doi: 10.1007/BF00273768.
Unbiased estimators of genotype and allele frequencies and their respective variances are obtained for loci identified by mendelian segregation in haploid female gametophytes from individual trees. By a minimum sampling variance criterion, the allocation of experimental effort between the number of female gametophytes analysed per tree and the number of trees sampled per population is examined for a fixed total amount of experimental effort. For estimating heterozygosity, the optimum sampling design for many (generally most) cases is three female gametophytes per tree, but may be more than three depending upon the true genotype frequencies in the population. For estimating allele frequencies, the optimum sampling design is one female gametophyte per tree except in cases where a strong negative correlation exists between alleles within genotpyes. Guidelines are discussed for determining a suitable number of female gametophytes to be analysed per tree in order to estimate heterozygosity.
在单倍体雌配子体中，通过孟德尔分离鉴定的基因座，可以获得基因型和等位基因频率及其各自方差的无偏估计值。通过最小抽样方差准则，在固定实验工作量的情况下，研究了每株树分析的雌配子体数量和每个群体采样的树数量之间的实验工作分配。对于估计杂合度，对于许多（通常是大多数）情况，最佳抽样设计是每株树三个雌配子体，但根据群体中真实的基因型频率，可能会超过三个。对于估计等位基因频率，最佳抽样设计是每株树一个雌配子体，除非基因型内的等位基因之间存在强烈的负相关。讨论了确定每株树要分析的合适数量的雌配子体以估计杂合度的指南。
