控制小麦及相关物种盐溶蛋白（白蛋白和球蛋白）的基因的染色体定位。

Chromosomal assignment of genes controlling salt-soluble proteins (albumins and globulins) in wheat and related species.

机构信息

Departamento de Bioquimíca, E.T.S. Ingenieros Agrónomos-UPM, 3, Madrid, Spain.

出版信息

Theor Appl Genet. 1984 Dec;69(2):167-72. doi: 10.1007/BF00272890.

Abstract

Salt-soluble proteins from the endosperms of wheat, barley, and rye have been separated by nonequilibrium electrofocusing x electrophoresis. Genes encoding 14 of the 25 components observed in wheat have been unambiguously assigned to 10 different chromosomes (1B, 3B, 3D, 4A, 4D, 5B, 6B, 6D, 7B, 7D) by analysis of the compensated nulli-tetrasomic series. Five more wheat proteins seem to be controlled by group 2 chromosomes. Analysis of wheat-barley and wheat-rye addition lines has led to the location of genes for 6 out of 20 barley proteins in 4 different chromosomes (1H, 3H, 4H, 6H; 1H is homoeologous to group 7 chromosomes of wheat) and of genes for 5 out of 20 rye proteins in two different chromosomes (2R, 4R). The relationship between the proteins reported here and previously characterized ones is discussed.

摘要

已通过非平衡等电聚焦 x 电泳分离出小麦、大麦和黑麦胚乳的盐溶性蛋白质。通过对补偿单体四体系列的分析，明确将观察到的 25 个成分中的 14 个编码基因分配到 10 条不同的染色体（1B、3B、3D、4A、4D、5B、6B、6D、7B、7D）上。另外 5 种小麦蛋白质似乎受 2 号染色体组控制。对小麦-大麦和小麦-黑麦添加系的分析导致在 4 条不同染色体（1H、3H、4H、6H；1H 与小麦第 7 染色体组同源）中定位了 20 种大麦蛋白质中的 6 种基因，以及在两条不同染色体（2R、4R）中定位了 20 种黑麦蛋白质中的 5 种基因。讨论了这里报道的蛋白质与以前表征的蛋白质之间的关系。