Departamento de Bioquímica, E.T.S. Ingenieros Agrónomos, Ciudad Universitaria, E-28040, Madrid, Spain.
Theor Appl Genet. 1987 Oct;74(6):811-6. doi: 10.1007/BF00247561.
Three proteins (components 1, 2, and 4) of the non-prolamin, 70% ethanol soluble fraction from the endosperm of Hordeum chilense have been identified as putative subunits of the tetrameric inhibitors active against insect α-amylases. In experiments carried out with the synthetic alloploid Tritordeum (H. chilense x Triticum turgidum conv. durum), previously described proteins from T. turgidum, designated CM2, CM3 and CM 16, have been also identified as subunits of α-amylase inhibitors. Genes for components 1 and 4 of H. chilense have been located in chromosomes 4H(ch) and 7H(ch), based on the analysis of H. chilense-T.turgidum addition lines. Subunits of the inhibitors from wheat and from cultivated barley had been previously assigned to chromosomes of the same homoeology groups.
三种非醇溶蛋白（1、2、4 组分）来源于大麦属杂种（Hordeum chilense×Triticum turgidum conv. durum）的醇溶蛋白部分，已经被鉴定为昆虫α-淀粉酶四聚体抑制剂的假定亚基。在以前的实验中，我们用人工合成的异源多倍体（H. chilense×Triticum turgidum conv. durum）进行实验，发现先前在 T. turgidum 中鉴定出的 CM2、CM3 和 CM16 蛋白质也被鉴定为α-淀粉酶抑制剂的亚基。基于对 H. chilense-T. turgidum 附加系的分析，已经将 H. chilense 的 1 号和 4 号组分的基因定位到 4H(ch)和 7H(ch)染色体上。先前已经将小麦和栽培大麦抑制剂的亚基分配到相同同源群的染色体上。
