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六倍体小麦、黑麦和大麦中叶过氧化物酶基因的染色体定位。

The chromosomal locations of leaf peroxidase genes in hexaploid wheat, rye and barley.

机构信息

Plant Breeding Institute, Maris Lane, CB2 2LQ, Trumpington, Cambridge, England.

出版信息

Theor Appl Genet. 1984 Dec;69(2):205-10. doi: 10.1007/BF00272896.

Abstract

Eight leaf peroxidase isozymes were distinguished by IEF in 'Chinese Spring'. Two genes which control the production of three of these isozymes were located on chromosome arms 1BS and 1DS by nullisomic analysis. These loci probably form part of a homoeoallelic series and have been designated Per-B1 and Per-D1 respectively. Analysis of chromosome 1B short arm terminal deletion stocks indicated that the Per-B1 locus is located between the nucleolar organiser region and another isozyme marker, Hk-B1. Two variant leaf peroxidase phenotypes were distinguished in a small sample of hexaploid wheat varieties. Analysis of wheat-alien addition and substitution lines identified homoeologous loci in rye (Per-R1) and barley (Per-H1).

摘要

在“中国春”中,通过等电聚焦区分了 8 种叶过氧化物同工酶。通过单体分析,确定控制其中 3 种同工酶产生的 2 个基因位于 1BS 和 1DS 染色体臂上。这些位点可能是同系等位基因系列的一部分,分别被指定为 Per-B1 和 Per-D1。对 1B 染色体短臂末端缺失品系的分析表明,Per-B1 位点位于核仁组织者区域和另一个同工酶标记 Hk-B1 之间。从小麦品种的一个小样本中区分出两种变体叶过氧化物酶表型。对小麦-外源添加和替代系的分析鉴定了黑麦(Per-R1)和大麦(Per-H1)中的同源基因座。

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