Dipartimento di Biologia Vegetale, Universitâ "La Sapienza", I-00165, Roma, Italy.
Plant Cell Rep. 1985 May;4(3):155-7. doi: 10.1007/BF00571305.
In this paper we describe a simple method to improve the plating efficiency in plant cell cultures.Two-stage plating is used; in the first stage the cells are inoculated at high density in 0.2% agarized culture medium for ten days to facilitate growth; under this condition, each cell produces a single micro-colony trapped in the agar network. In the second stage the colonies are plated at different densities in 1% agarized medium.These colonies are self-sufficient and able to improve the cell growth by conditioning the medium.
本文介绍了一种提高植物细胞培养中接种效率的简单方法。采用两阶段接种法；第一阶段在 0.2%琼脂化培养基中以高细胞密度接种 10 天，以促进细胞生长；在此条件下，每个细胞产生一个被困在琼脂网络中的单个微菌落。第二阶段在 1%琼脂化培养基中以不同密度对菌落进行接种。这些菌落自给自足，能够通过调节培养基来改善细胞生长。
