Department of Microbiology, University of Illinois, Urbana, Illinois 61801.
Appl Environ Microbiol. 1983 Jul;46(1):220-6. doi: 10.1128/aem.46.1.220-226.1983.
Plating techniques for cultivation of methanogenic bacteria have been optimized for two members of the genus Methanococcus. Methanococcus maripaludis and Methanococcus voltae were cultivated on aerobically and anaerobically prepared agar media. Maintenance of O(2) levels below 5 mul/liter within an anaerobic glove box was necessary during plating and incubation for 90% recovery of plated cells. Under an atmosphere of H(2), CO(2), and H(2)S (79:20:1), 2 to 3 days of incubation at 37 degrees C were sufficient for the formation of visible colonies. The viability of plated cells was significantly affected by the growth phase of the culture, H(2)S concentration, and the volume of medium per plate. In addition, colony size of methanococci was affected by agar type, as well as by the concentrations of agar, H(2)S, and bicarbonate.
已针对产甲烷菌属的两个成员对培养产甲烷菌的 plating 技术进行了优化。在有氧和无氧准备的琼脂培养基上培养 Methanococcus maripaludis 和 Methanococcus voltae。在接种和孵育过程中，需要在厌氧手套箱内将 O(2)水平保持在 5 μl/L 以下，以实现 90%的 plated 细胞回收。在 H(2)、CO(2)和 H(2)S（79:20:1）的气氛下，在 37°C 下孵育 2 到 3 天即可形成可见菌落。 plated 细胞的存活率受培养物的生长阶段、H(2)S 浓度和每个平板中的培养基体积的显著影响。此外，甲烷球菌的菌落大小受琼脂类型以及琼脂、H(2)S 和碳酸氢盐的浓度影响。
