Kumlehn J, Schieder O, Lörz H
Centre for Applied Plant Molecular Biology (AMP II), University of Hamburg, Ohnhorststraße 18, D-22609 Hamburg, Germany Fax no.: +49-40-82282-229 E-mail:
Institute of Applied Genetics, Free University of Berlin, Albrecht-Thaer-Weg 6, D-14195 Berlin, Germany, , , , , , DE.
Plant Cell Rep. 1997 Jul;16(10):663-667. doi: 10.1007/s002990050298.
Ovules of the wheat breeding line Veery #5 were excised and transferred to culture within 24 h after pollination. When ovules were cultured on Phytagel-solidified medium, and the pericarp removed exclusively at the micropylar tip and the abaxial side, zygotes from up to 79.2% of the ovules underwent embryogenesis with the same developmental pattern as found in planta. Embryos from more than 50% of the cultured ovules germinated when transferred to regeneration medium. More than 100 plantlets were randomly chosen for transfer to soil, all of which developed to phenotypically normal and fertile plants. With this system, the entire process of zygotic embryogenesis can be studied using living material. Furthermore, the method could be used as an embryo rescue technique for plant breeding purposes.
小麦育种系Veery #5的胚珠在授粉后24小时内被切除并转移到培养基中培养。当胚珠在植物凝胶固化培养基上培养，且仅在珠孔端和背面去除果皮时，高达79.2%的胚珠中的合子经历胚胎发生，其发育模式与在植株中发现的相同。当转移到再生培养基上时，超过50%的培养胚珠中的胚胎发芽。随机选择100多株幼苗转移到土壤中，所有这些幼苗都发育成表型正常且可育的植株。利用这个系统，可以使用活体材料研究合子胚胎发生的整个过程。此外，该方法可用作植物育种目的的胚胎拯救技术。
