番茄原生质体的全能性。
Totipotency of tomato protoplasts.
机构信息
Genetics and Tissue Culture Group, ARCO Plant Cell Research Institute, 6560 Trinity Court, 94568, Dublin, CA, USA.
出版信息
Theor Appl Genet. 1985 May;69(3):235-40. doi: 10.1007/BF00662431.
An efficient and reliable protocol for tomato protoplast isolation, culture, and plant regeneration has been developed. Fourteen diverse cultivars were tested. Fertile plants were regenerated from all 14 cultivars without any modification in the protocol. Plating efficiency (percentage of the protoplasts that formed minicalli) of up to 50% was achieved. Those mini-calli rapidly regenerated shoots at high frequencies. Regenerated shoots can be easily rooted on a basal medium with the appropriate auxin, and have been set to soil within two months after the isolation of the protoplasts.
已经开发出一种高效可靠的番茄原生质体分离、培养和植株再生的方案。对 14 个不同品种进行了测试。通过该方案，无需任何修改，即可从所有 14 个品种中再生出可育植株。平板效率（形成小型愈伤组织的原生质体百分比）高达 50%。这些小型愈伤组织可快速高频再生出芽。再生芽可在含有适当生长素的基础培养基上轻松生根，并在原生质体分离后两个月内移栽到土壤中。
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