Department of Botany, University of Delhi, 110 007, Delhi, India.
Plant Cell Rep. 1985 Aug;4(4):191-4. doi: 10.1007/BF00269286.
Somatic embryogenesis leading to plant regeneration has been achieved in the bamboo, Dendrocalamus strictus, by culturing seeds (caryopses) on B5 basal medium supplemented with 2,4-dichlorophenoxyacetic acid. Callus cultures obtained from the embryonal end of the seeds differentiated chlorophyllous embryoids. On transfer to a germination medium (B5 liquid, sucrose, indolebutyric acid, and ∝ -naphthaleneacetic acid) 40% of the embryoids developed into plantlets. Further development of the plantlets occured on B5 liquid medium (half strength) + sucrose (1%) + IBA (5 × 10(-7)M) + NAA (10(-7)M).
体细胞胚胎发生导致植物再生已在竹子，麻竹，通过培养种子（子实）上 B5 基本培养基补充 2,4-二氯苯氧乙酸。从种子的胚端获得的愈伤组织分化出含叶绿素的胚状体。转移到发芽培养基（B5 液体、蔗糖、吲哚丁酸和α-萘乙酸）上后，40%的胚状体发育成小植株。小植株在 B5 液体培养基（半强度）+蔗糖（1%）+IBA（5×10（-7）M）+NAA（10（-7）M）上进一步发育。
