Ye Shanwen, Cai Changyang, Ren Huibo, Wang Wenjia, Xiang Mengqi, Tang Xiaoshan, Zhu Caiping, Yin Tengfei, Zhang Li, Zhu Qiang
Basic Forestry and Proteomics Center, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Haixia Institute of Science and Technology, College of Forestry, Fujian Agriculture and Forestry UniversityFuzhou, China.
Front Plant Sci. 2017 Jul 27;8:1298. doi: 10.3389/fpls.2017.01298. eCollection 2017.
Genetic engineering technology has been successfully used in many plant species, but is limited in woody plants, especially in bamboos. Ma bamboo ( Munro) is one of the most important bamboo species in Asia, and its genetic improvement was largely restricted by the lack of an efficient regeneration and transformation method. Here we reported a plantlet regeneration and -mediated transformation protocol by using Ma bamboo young shoots as explants. Under our optimized conditions, embryogenic calluses were successfully induced from the excised young shoots on callus induction medium and rapidly grew on callus multiplication medium. Shoots and roots were regenerated on shoot induction medium and root induction medium, respectively, with high efficiency. An -mediated genetic transformation protocol of Ma bamboo was established, verified by PCR and GUS staining. Furthermore, the maize gene under the control of the ubiquitin promoter was successfully introduced into Ma bamboo genome and generated an anthocyanin over-accumulation phenotype. Our methods established here will facilitate the basic research as well as genetic breeding of this important bamboo species. : A stable and high efficiency regeneration and -mediated transformation protocol for Ma bamboo from vegetative organ is established.
基因工程技术已在许多植物物种中成功应用，但在木本植物中受到限制，尤其是在竹子中。麻竹（Munro）是亚洲最重要的竹种之一，其遗传改良在很大程度上受到缺乏高效再生和转化方法的限制。在此，我们报道了一种以麻竹嫩梢为外植体的植株再生和介导转化方案。在我们优化的条件下，从愈伤组织诱导培养基上切下的嫩梢成功诱导出胚性愈伤组织，并在愈伤组织增殖培养基上快速生长。芽和根分别在芽诱导培养基和根诱导培养基上高效再生。建立了麻竹的介导遗传转化方案，并通过PCR和GUS染色进行了验证。此外，泛素启动子控制下的玉米基因成功导入麻竹基因组，并产生花青素过度积累表型。我们在此建立的方法将有助于对这种重要竹种进行基础研究以及遗传育种。：建立了一种从营养器官出发的麻竹稳定高效再生和介导转化方案。
