Plant Genetic Engineering Laboratory and Department of Horticulture, New Mexico State University, 88003, Las Cruces, NM, USA.
Plant Cell Rep. 1987 Apr;6(2):105-8. doi: 10.1007/BF00276664.
A successful Agrobacterium-mediated transformation system involving a disarmed Ti plasmid is composed of two stages: transformation of cells and recovery of transformed plants. A tissue transformation system with 34% efficiency was developed using stem segments of the interspecific tomato hybrid Lycopersicon esculentum × L. pennellii. This transformation system emphasizes three factors favoring the recovery of transformed plants: 1) promotion of cell division activity at the inoculation site with kinetin in the incubation medium, 2) promotion of adventitious bud initiation by using organized tissue explants in culture, and 3) application of selection at the shoot development stage of adventitious regeneration.
一个成功的农杆菌介导转化系统，涉及一种无武装的 Ti 质粒，由两个阶段组成：细胞转化和转化植物的恢复。使用番茄种间杂种 Lycopersicon esculentum × L. pennellii 的茎段，开发了一种具有 34%效率的组织转化系统。该转化系统强调了有利于转化植物恢复的三个因素：1）在培养物中用激动素促进接种部位的细胞分裂活性，2）通过在培养中使用组织外植体促进不定芽的起始，3）在不定芽再生的芽发育阶段应用选择。
