Laboratorium voor Plantenbiochemie, Katholieke Universiteit Leuven, Vaartstraat 24, B-3000, Leuven, Belgium.
Planta. 1982 Jun;154(6):562-7. doi: 10.1007/BF00403001.
Wheat (Triticum aestivum) germ agglutinin represents a complex mixture of multiple isolectin forms. Upon ion exchange chromatography at pH 3.8, three isolectins can be separated, each of which is composed of two identical subunits. At pH 5.0, however, three additional isolectins can be distinguished, which are built up of two different subunits (heteromeric lectins). Evidence is presented that these heterodimers are normal constituents of the wheat embryo cells. Analyses of the isolectin patterns in extracts from Triticum monococcum, Triticum turgidum dicoccum and Triticum aestivum, provide evidence that each genome, either in simple or complex (polyploid) genomes, directs the synthesis of a single lectin subunit species. In addition, a comparison of the isolectin pattern in these wheat species of increasing ploidy level, made it possible to determine unequivocally the genome by which the individual lectin subunits in polyploid species are coded for. The possible use of lectins in studies on the origin of individual genoms in polyploid species is discussed.
小麦胚芽凝集素是由多种同功凝集素形式组成的复杂混合物。在 pH3.8 进行离子交换层析时，可以分离出三种同功凝集素，它们各自由两个相同的亚基组成。然而，在 pH5.0 时，可以区分出另外三种同功凝集素，它们由两个不同的亚基（异源二聚体凝集素）组成。有证据表明，这些异源二聚体是小麦胚细胞的正常成分。对从小麦单胞体、小麦硬质二粒小麦和小麦中提取的同功凝集素模式的分析表明，每个基因组，无论是简单的还是复杂的（多倍体）基因组，都指导一种单一的凝集素亚基物种的合成。此外，对这些小麦物种中同功凝集素模式的比较，使得在多倍体物种中确定个体凝集素亚基所编码的基因组变得可能。讨论了凝集素在研究多倍体物种中个别基因组起源中的可能用途。
