Lamb J E, Shibata S, Goldstein I J
Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109.
Plant Physiol. 1983 Apr;71(4):879-87. doi: 10.1104/pp.71.4.879.
Leaves from mature Griffonia simplicifolia plants were examined for the presence of leaf lectins possessing sugar binding specificities similar to the four known seed lectins (GS-I, GS-II, GS-III, GS-IV). Three (GS-I, -II, -IV) of the four known G. simplicifolia seed lectins were present in the leaves. Leaf G. simplicifolia lectins I and IV were similar to the respective seed lectins. Leaf GS-II, however, was composed of two types of subunits (M(r) = 33,000 and 19,000), whereas the seed lectin consists of only one type of subunit (M(r) 32,500). Seed and leaf GS-II lectins also had different isoelectric points. All leaf and seed lectins were similar with respect to their hemagglutination and glycoconjugate precipitation properties and all subunits contained covalently bound carbohydrate. Leaf GS-IV appeared slightly under-glycosylated compared to seed GS-IV.The fate of GS-I and GS-II seed lectins in aging cotyledons was investigated. GS-I isolectins usually contain isolectin subtypes associated with each main isolectin. Upon inbibition and germination, these GS-I isolectin subtypes disappeared. Over time, GS-II lectin did not change its disc gel electrophoretic properties.
对成熟的西非刺桐植物叶片进行检查，以确定是否存在具有与四种已知种子凝集素（GS-I、GS-II、GS-III、GS-IV）相似的糖结合特异性的叶片凝集素。四种已知的西非刺桐种子凝集素中的三种（GS-I、-II、-IV）存在于叶片中。叶片中的西非刺桐凝集素I和IV与各自的种子凝集素相似。然而，叶片GS-II由两种亚基组成（相对分子质量分别为33,000和19,000），而种子凝集素仅由一种亚基组成（相对分子质量32,500）。种子和叶片的GS-II凝集素也具有不同的等电点。所有叶片和种子凝集素在血凝和糖缀合物沉淀特性方面相似，并且所有亚基都含有共价结合的碳水化合物。与种子GS-IV相比，叶片GS-IV的糖基化程度似乎略低。研究了GS-I和GS-II种子凝集素在衰老子叶中的命运。GS-I同工凝集素通常包含与每种主要同工凝集素相关的同工凝集素亚型。在吸水和萌发过程中，这些GS-I同工凝集素亚型消失。随着时间的推移，GS-II凝集素的圆盘凝胶电泳特性没有改变。
