Department of Botany, University of Massachusetts, 01003, Amherst, MA, USA.
Theor Appl Genet. 1985 Mar;69(5-6):597-602. doi: 10.1007/BF00251110.
The purpose of this paper was to determine if heavy metal tolerance was expressed in pollen and if its expression was correlated with the tolerance of the pollen source. Clones of Silene dioica, tolerant to zinc, closely related but nontolerant S. alba and clones of Mimulus guttatus tolerant and sensitive to copper were grown in the greenhouse in either standard potting soil or nutrient culture. Pollen was collected shortly after dehiscence, hydrated, and tested over a broad range of metal concentrations. The tolerance of the pollen source was determined by comparing root growth in solutions with and without heavy metals. In both Silene species and M. guttatus, the tolerance of the parental clone was expressed in its pollen. Pollen from tolerant individuals was able to germinate and grow at concentrations of metals which markedly inhibited pollen from nontolerants.
本文旨在探讨花粉是否表达重金属耐受性，如果表达，其表达与花粉源的耐受性是否相关。在温室中，以标准盆栽土或营养培养的方式种植耐受锌的野菀克隆种、亲缘关系密切但不耐锌的野菀的非耐受克隆种以及耐受和敏感铜的金鱼草克隆种。花粉在散粉后不久收集，水合后在广泛的金属浓度范围内进行测试。花粉源的耐受性通过比较有和没有重金属的溶液中的根生长来确定。在野菀属和金鱼草属中，亲代克隆的耐受性在其花粉中表达。耐受个体的花粉能够在明显抑制非耐受个体花粉的金属浓度下萌发和生长。
